Influence of TNF and IL10 gene polymorphisms in the immunopathogenesis of leprosy in the south of Brazil.

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TitleInfluence of TNF and IL10 gene polymorphisms in the immunopathogenesis of leprosy in the south of Brazil.
Publication TypeJournal Article
AuthorsFranceschi DSA, Mazini PS, Rudnick CCC, Sell AM, Tsuneto LT, Ribas ML, Peixoto PR, Visentainer JEL
Abbrev. JournalInt. J. Infect. Dis.
JournalInternational journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases
Year of Publication2009
Publication Languageeng
KeywordsBrazil, Ethnic Groups, Female, Gene Frequency, Genotype, Humans, Interleukin-10, Leprosy, Male, Polymerase Chain Reaction, Polymorphism, Genetic, Polymorphism, Single Nucleotide, Reference Values, Tumor Necrosis Factor-alpha

OBJECTIVE: To determine whether cytokine polymorphisms are associated with leprosy and/or their subtypes in a Brazilian population.

METHODS: Genotyping using polymerase chain reaction with sequence-specific primers (PCR-SSP) was performed for: TNF(-308/-238), IL2(-330/+166), IL6(-174), IFNG(+874), TGFB1(+869/+915), and IL10(-592/-819/-1082) in 240 healthy controls and 167 patients with leprosy.

RESULTS: For TNF(-308), a higher frequency of GG genotype (85.5% vs. 74.1% in healthy controls, p = 0.009), along with a decreased frequency of GA/AA genotypes was observed among leprosy patients as compared to the control group (14.5% vs. 25.9%, p = 0.009). The GG genotype was particularly higher in patients with tuberculoid (TT) and borderline (BB) leprosy (90.5% and 89.8%, respectively). Analysis of IL10 genotypes revealed a lower frequency of GCC/GCC haplotype in lepromatous leprosy (LL) patients (6.2%) in comparison to controls (15.4%).

CONCLUSION: It is suggested that the G-->A substitution at position -308 in the TNF promoter region plays an important role in leprosy patients.

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