@article{81,
  keywords = {Brazil, Ethnic Groups, Female, Gene Frequency, genotype, Humans, Interleukin-10, leprosy, Male, polymerase chain reaction, Polymorphism, Genetic, Polymorphism, Single Nucleotide, Reference Values, Tumor Necrosis Factor-alpha},
  author = {Franceschi DSA and Mazini PS and Rudnick CCC and Sell AM and Tsuneto LT and Ribas ML and Peixoto PR and Visentainer JEL},
  title = {Influence of TNF and IL10 gene polymorphisms in the immunopathogenesis of leprosy in the south of Brazil.},
  abstract = {<p><b>OBJECTIVE: </b>To determine whether cytokine polymorphisms are associated with leprosy and/or their subtypes in a Brazilian population.</p><p><b>METHODS: </b>Genotyping using polymerase chain reaction with sequence-specific primers (PCR-SSP) was performed for: TNF(-308/-238), IL2(-330/+166), IL6(-174), IFNG(+874), TGFB1(+869/+915), and IL10(-592/-819/-1082) in 240 healthy controls and 167 patients with leprosy.</p><p><b>RESULTS: </b>For TNF(-308), a higher frequency of GG genotype (85.5% vs. 74.1% in healthy controls, p = 0.009), along with a decreased frequency of GA/AA genotypes was observed among leprosy patients as compared to the control group (14.5% vs. 25.9%, p = 0.009). The GG genotype was particularly higher in patients with tuberculoid (TT) and borderline (BB) leprosy (90.5% and 89.8%, respectively). Analysis of IL10 genotypes revealed a lower frequency of GCC/GCC haplotype in lepromatous leprosy (LL) patients (6.2%) in comparison to controls (15.4%).</p><p><b>CONCLUSION: </b>It is suggested that the G-->A substitution at position -308 in the TNF promoter region plays an important role in leprosy patients.</p>},
  year = {2009},
  journal = {International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases},
  volume = {13},
  pages = {493-8},
  month = {2009 Jul},
  issn = {1878-3511},
  doi = {10.1016/j.ijid.2008.08.019},
  language = {eng},
}