TY - JOUR
KW - Brazil
KW - Ethnic Groups
KW - Female
KW - Gene Frequency
KW - genotype
KW - Humans
KW - Interleukin-10
KW - leprosy
KW - Male
KW - polymerase chain reaction
KW - Polymorphism, Genetic
KW - Polymorphism, Single Nucleotide
KW - Reference Values
KW - Tumor Necrosis Factor-alpha
AU - Franceschi DSA
AU - Mazini PS
AU - Rudnick CCC
AU - Sell AM
AU - Tsuneto LT
AU - Ribas ML
AU - Peixoto PR
AU - Visentainer JEL
AB - <p><b>OBJECTIVE: </b>To determine whether cytokine polymorphisms are associated with leprosy and/or their subtypes in a Brazilian population.</p><p><b>METHODS: </b>Genotyping using polymerase chain reaction with sequence-specific primers (PCR-SSP) was performed for: TNF(-308/-238), IL2(-330/+166), IL6(-174), IFNG(+874), TGFB1(+869/+915), and IL10(-592/-819/-1082) in 240 healthy controls and 167 patients with leprosy.</p><p><b>RESULTS: </b>For TNF(-308), a higher frequency of GG genotype (85.5% vs. 74.1% in healthy controls, p = 0.009), along with a decreased frequency of GA/AA genotypes was observed among leprosy patients as compared to the control group (14.5% vs. 25.9%, p = 0.009). The GG genotype was particularly higher in patients with tuberculoid (TT) and borderline (BB) leprosy (90.5% and 89.8%, respectively). Analysis of IL10 genotypes revealed a lower frequency of GCC/GCC haplotype in lepromatous leprosy (LL) patients (6.2%) in comparison to controls (15.4%).</p><p><b>CONCLUSION: </b>It is suggested that the G-->A substitution at position -308 in the TNF promoter region plays an important role in leprosy patients.</p>
BT - International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases
C1 - http://www.ncbi.nlm.nih.gov/pubmed/19058987?dopt=Abstract
DA - 2009 Jul
DO - 10.1016/j.ijid.2008.08.019
IS - 4
J2 - Int. J. Infect. Dis.
LA - eng
N2 - <p><b>OBJECTIVE: </b>To determine whether cytokine polymorphisms are associated with leprosy and/or their subtypes in a Brazilian population.</p><p><b>METHODS: </b>Genotyping using polymerase chain reaction with sequence-specific primers (PCR-SSP) was performed for: TNF(-308/-238), IL2(-330/+166), IL6(-174), IFNG(+874), TGFB1(+869/+915), and IL10(-592/-819/-1082) in 240 healthy controls and 167 patients with leprosy.</p><p><b>RESULTS: </b>For TNF(-308), a higher frequency of GG genotype (85.5% vs. 74.1% in healthy controls, p = 0.009), along with a decreased frequency of GA/AA genotypes was observed among leprosy patients as compared to the control group (14.5% vs. 25.9%, p = 0.009). The GG genotype was particularly higher in patients with tuberculoid (TT) and borderline (BB) leprosy (90.5% and 89.8%, respectively). Analysis of IL10 genotypes revealed a lower frequency of GCC/GCC haplotype in lepromatous leprosy (LL) patients (6.2%) in comparison to controls (15.4%).</p><p><b>CONCLUSION: </b>It is suggested that the G-->A substitution at position -308 in the TNF promoter region plays an important role in leprosy patients.</p>
PY - 2009
SP - 493
EP - 8
T2 - International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases
TI - Influence of TNF and IL10 gene polymorphisms in the immunopathogenesis of leprosy in the south of Brazil.
VL - 13
SN - 1878-3511
ER -