02122nas a2200385 4500000000100000008004100001260001300042653001100055653001800066653001100084653001900095653001300114653001100127653001900138653001200157653000900169653003000178653002600208653003600234653002100270653003200291100001900323700001400342700001600356700001200372700001500384700001300399700001500412700002000427245011000447300001000557490000700567520114800574022001401722 2009 d c2009 Jul10aBrazil10aEthnic Groups10aFemale10aGene Frequency10agenotype10aHumans10aInterleukin-1010aleprosy10aMale10apolymerase chain reaction10aPolymorphism, Genetic10aPolymorphism, Single Nucleotide10aReference Values10aTumor Necrosis Factor-alpha1 aFranceschi DSA1 aMazini PS1 aRudnick CCC1 aSell AM1 aTsuneto LT1 aRibas ML1 aPeixoto PR1 aVisentainer JEL00aInfluence of TNF and IL10 gene polymorphisms in the immunopathogenesis of leprosy in the south of Brazil. a493-80 v133 a

OBJECTIVE: To determine whether cytokine polymorphisms are associated with leprosy and/or their subtypes in a Brazilian population.

METHODS: Genotyping using polymerase chain reaction with sequence-specific primers (PCR-SSP) was performed for: TNF(-308/-238), IL2(-330/+166), IL6(-174), IFNG(+874), TGFB1(+869/+915), and IL10(-592/-819/-1082) in 240 healthy controls and 167 patients with leprosy.

RESULTS: For TNF(-308), a higher frequency of GG genotype (85.5% vs. 74.1% in healthy controls, p = 0.009), along with a decreased frequency of GA/AA genotypes was observed among leprosy patients as compared to the control group (14.5% vs. 25.9%, p = 0.009). The GG genotype was particularly higher in patients with tuberculoid (TT) and borderline (BB) leprosy (90.5% and 89.8%, respectively). Analysis of IL10 genotypes revealed a lower frequency of GCC/GCC haplotype in lepromatous leprosy (LL) patients (6.2%) in comparison to controls (15.4%).

CONCLUSION: It is suggested that the G-->A substitution at position -308 in the TNF promoter region plays an important role in leprosy patients.

 a1878-3511