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Publication

Mycobacterium leprae isolates from different sources have identical sequences of the spacer region between the 16S and 23S ribosomal RNA genes.

Abstract

To test for genotypic variations between different isolates of Mycobacterium leprae, the causative agent of leprosy, the 282 bp spacer region between the 16S and 23S rRNA genes was amplified using PCR, and submitted to single-strand conformation polymorphism (SSCP) analysis. The procedure was optimized using four modified spacer fragments, containing mutations at one, three, four and six positions, respectively. Seventy-five M. leprae isolates from different sources, including isolates from leprosy patients, healthy individuals, armadillos and mouse footpads were identical in the SSCP analysis. DNA sequencing and restriction enzyme analysis performed on four and 40 samples, respectively, confirmed the results obtained with SSCP analysis.

More information

Type
Journal Article
Author
Wit M Y
Klatser P R
Year of Publication
1994
Journal
Microbiology (Reading, England)
Volume
140 ( Pt 8)
Number of Pages
1983-7
Date Published
1994 Aug
Language
eng
ISSN Number
1350-0872
DOI
10.1099/13500872-140-8-1983
Alternate Journal
Microbiology (Reading, Engl.)
Publication Language
eng