TY - JOUR KW - Animals KW - Armadillos KW - Base Sequence KW - Cercocebus atys KW - DNA, Bacterial KW - DNA, Ribosomal KW - Genes, Bacterial KW - Humans KW - leprosy KW - Mice KW - Mice, Nude KW - Monkey Diseases KW - Mycobacterium leprae KW - polymerase chain reaction KW - Polymorphism, Genetic KW - Polymorphism, Single-Stranded Conformational KW - Restriction Mapping KW - RNA, Bacterial KW - RNA, Ribosomal, 16S KW - RNA, Ribosomal, 23S KW - Sequence Homology, Nucleic Acid AU - Wit M Y AU - Klatser P R AB -

To test for genotypic variations between different isolates of Mycobacterium leprae, the causative agent of leprosy, the 282 bp spacer region between the 16S and 23S rRNA genes was amplified using PCR, and submitted to single-strand conformation polymorphism (SSCP) analysis. The procedure was optimized using four modified spacer fragments, containing mutations at one, three, four and six positions, respectively. Seventy-five M. leprae isolates from different sources, including isolates from leprosy patients, healthy individuals, armadillos and mouse footpads were identical in the SSCP analysis. DNA sequencing and restriction enzyme analysis performed on four and 40 samples, respectively, confirmed the results obtained with SSCP analysis.

BT - Microbiology (Reading, England) C1 - http://www.ncbi.nlm.nih.gov/pubmed/7522845?dopt=Abstract DA - 1994 Aug DO - 10.1099/13500872-140-8-1983 J2 - Microbiology (Reading, Engl.) LA - eng N2 -

To test for genotypic variations between different isolates of Mycobacterium leprae, the causative agent of leprosy, the 282 bp spacer region between the 16S and 23S rRNA genes was amplified using PCR, and submitted to single-strand conformation polymorphism (SSCP) analysis. The procedure was optimized using four modified spacer fragments, containing mutations at one, three, four and six positions, respectively. Seventy-five M. leprae isolates from different sources, including isolates from leprosy patients, healthy individuals, armadillos and mouse footpads were identical in the SSCP analysis. DNA sequencing and restriction enzyme analysis performed on four and 40 samples, respectively, confirmed the results obtained with SSCP analysis.

PY - 1994 SP - 1983 EP - 7 T2 - Microbiology (Reading, England) TI - Mycobacterium leprae isolates from different sources have identical sequences of the spacer region between the 16S and 23S ribosomal RNA genes. VL - 140 ( Pt 8) SN - 1350-0872 ER -