01877nas a2200397 4500000000100000008004100001260001300042653001200055653001500067653001800082653002000100653001900120653001900139653002100158653001100179653001200190653000900202653001500211653002000226653002500246653003000271653002600301653004900327653002400376653001900400653002400419653002400443653003600467100001200503700001600515245014800531300001100679490001600690520075900706022001401465 1994 d c1994 Aug10aAnimals10aArmadillos10aBase Sequence10aCercocebus atys10aDNA, Bacterial10aDNA, Ribosomal10aGenes, Bacterial10aHumans10aleprosy10aMice10aMice, Nude10aMonkey Diseases10aMycobacterium leprae10apolymerase chain reaction10aPolymorphism, Genetic10aPolymorphism, Single-Stranded Conformational10aRestriction Mapping10aRNA, Bacterial10aRNA, Ribosomal, 16S10aRNA, Ribosomal, 23S10aSequence Homology, Nucleic Acid1 aWit M Y1 aKlatser P R00aMycobacterium leprae isolates from different sources have identical sequences of the spacer region between the 16S and 23S ribosomal RNA genes. a1983-70 v140 ( Pt 8)3 a
To test for genotypic variations between different isolates of Mycobacterium leprae, the causative agent of leprosy, the 282 bp spacer region between the 16S and 23S rRNA genes was amplified using PCR, and submitted to single-strand conformation polymorphism (SSCP) analysis. The procedure was optimized using four modified spacer fragments, containing mutations at one, three, four and six positions, respectively. Seventy-five M. leprae isolates from different sources, including isolates from leprosy patients, healthy individuals, armadillos and mouse footpads were identical in the SSCP analysis. DNA sequencing and restriction enzyme analysis performed on four and 40 samples, respectively, confirmed the results obtained with SSCP analysis.
a1350-0872