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Publication

Surface antigens of Leishmania donovani promastigotes.

Abstract

Surface antigen profiles of Leishmania donovani promastigote isolates have been studied. Surface patterns of Brazilian and African isolates display remarkable similarities and are extremely simple, consisting of three major peptides of 65,000, 25,000, and 23,000 mol wt. Surface iodination and biosynthetic labeling coupled to immunoprecipitation techniques revealed that a single major determinant of 65,000 mol wt is recognized in all strains by sera from kala-azar patients from both Brazil and Africa. This major determinant is not brought down by sera from normal individuals and shows no significant cross-reactivity with sera from Chagas' disease, leprosy, or syphilis patients. Binding to concanavalin A suggests a glycoprotein nature for this antigen. Sera from patients with cutaneous leishmaniasis (L. braziliensis) also recognized the same 65,000-mol wt determinant, although to a lesser extent. The possibility that this major surface antigen is shared, with minor differences, not only by L. donovani strains but between Leishmania species in general is suggested.

More information

Type
Journal Article
Author
Lepay D A
Nogueira N
Cohn Z
Year of Publication
1983
Journal
The Journal of experimental medicine
Volume
157
Issue
5
Number of Pages
1562-72
Date Published
1983 May 01
Language
eng
ISSN Number
0022-1007
DOI
10.1084/jem.157.5.1562
Alternate Journal
J. Exp. Med.
Publication Language
eng