01769nas a2200301 4500000000100000008004100001260001600042653001200058653002200070653001100092653002000103653000900123653001100132653001500143653002800158653002100186653001300207653001400220653001000234100001400244700001500258700001100273245005900284300001200343490000800355520109000363022001401453 1983 d c1983 May 0110aAnimals10aAntigens, Surface10aBrazil10aCross Reactions10aDogs10aHumans10aLeishmania10aLeishmaniasis, Visceral10aMolecular Weight10aPeptides10aSepharose10aSudan1 aLepay D A1 aNogueira N1 aCohn Z00aSurface antigens of Leishmania donovani promastigotes. a1562-720 v1573 a
Surface antigen profiles of Leishmania donovani promastigote isolates have been studied. Surface patterns of Brazilian and African isolates display remarkable similarities and are extremely simple, consisting of three major peptides of 65,000, 25,000, and 23,000 mol wt. Surface iodination and biosynthetic labeling coupled to immunoprecipitation techniques revealed that a single major determinant of 65,000 mol wt is recognized in all strains by sera from kala-azar patients from both Brazil and Africa. This major determinant is not brought down by sera from normal individuals and shows no significant cross-reactivity with sera from Chagas' disease, leprosy, or syphilis patients. Binding to concanavalin A suggests a glycoprotein nature for this antigen. Sera from patients with cutaneous leishmaniasis (L. braziliensis) also recognized the same 65,000-mol wt determinant, although to a lesser extent. The possibility that this major surface antigen is shared, with minor differences, not only by L. donovani strains but between Leishmania species in general is suggested.
a0022-1007