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Publication

Polymerase chain reaction for the detection of Mycobacterium leprae.

Abstract

A polymerase chain reaction (PCR) using heat-stable Taq polymerase is described for the specific detection of Mycobacterium leprae, the causative agent of leprosy. A set of primers was selected on the basis of the nucleotide sequence of a gene encoding the 36 kDa antigen of M. leprae. With this set of primers in the PCR, M. leprae could be detected specifically with a detection limit approximating one bacterium. This PCR appears to meet the criteria of specificity and sensitivity required for a useful tool in epidemiology and eventually for the control of leprosy.

More information

Type
Journal Article
Author
Hartskeerl R A
Wit M Y
Klatser P R
Year of Publication
1989
Journal
Journal of general microbiology
Volume
135
Issue
9
Number of Pages
2357-64
Date Published
1989 Sep
Language
eng
ISSN Number
0022-1287
DOI
10.1099/00221287-135-9-2357
Alternate Journal
J. Gen. Microbiol.
Publication Language
eng

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