@article{10056,
  keywords = {Animals, Armadillos, Blotting, Southern, DNA-Directed DNA Polymerase, Electrophoresis, Agar Gel, leprosy, Liver, Mycobacterium leprae, Nucleic Acid Amplification Techniques, polymerase chain reaction},
  author = {Hartskeerl R A and Wit M Y and Klatser P R},
  title = {Polymerase chain reaction for the detection of Mycobacterium leprae.},
  abstract = {<p>A polymerase chain reaction (PCR) using heat-stable Taq polymerase is described for the specific detection of Mycobacterium leprae, the causative agent of leprosy. A set of primers was selected on the basis of the nucleotide sequence of a gene encoding the 36 kDa antigen of M. leprae. With this set of primers in the PCR, M. leprae could be detected specifically with a detection limit approximating one bacterium. This PCR appears to meet the criteria of specificity and sensitivity required for a useful tool in epidemiology and eventually for the control of leprosy.</p>},
  year = {1989},
  journal = {Journal of general microbiology},
  volume = {135},
  pages = {2357-64},
  month = {1989 Sep},
  issn = {0022-1287},
  url = {http://mic.microbiologyresearch.org/content/journal/micro/10.1099/00221287-135-9-2357},
  doi = {10.1099/00221287-135-9-2357},
  language = {eng},
}