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Extraction and detection of Mycobacterium leprae DNA from ZNCF-stained skin smear slides for better identification of negative skin smears.

Abstract

Abstract Identification of Mycobacterium leprae, which causes leprosy, is done by Ziehl Neelsen Carbol Fuchsin (ZNCF) stained slit skin smear microscopy that aids in the diagnosis and quantification of approximate bacterial load carried by the patient. We attempted M. leprae DNA extraction from 46 stained slit skin smear negative slides, using Proteinase K and SDS lysis, followed by ethanol precipitation. M. leprae specific primers (16SrRNA) were used for PCR-based amplification of DNA. We could detect M. leprae DNA in 15 (32.6%) samples. The method can be useful in the diagnosis of apparently slit skin smear negative leprosy cases.

More information

Type
Journal Article
Author
Kamble R R
Shinde V S
Madhale S P
Kamble A A
Ravikumar B P
Jadhav R S
Year of Publication
2010
Journal
Indian journal of medical microbiology
Volume
28
Issue
1
Number of Pages
57-9
Date Published
2010 Jan-Mar
Language
eng
ISSN Number
1998-3646
DOI
10.4103/0255-0857.58732
Alternate Journal
Indian J Med Microbiol
Publication Language
eng

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