01645nas a2200361   4500000000100000008004100001260001700042653001500059653001600074653001900090653002000109653001100129653001200140653002500152653002500177653003000202653001900232653002400251653000900275653002700284100001500311700001500326700001600341700001500357700001800372700001500390245014400405856005200549300000900601490000700610520065200617022001401269       2010                            d  c2010 Jan-Mar10aDetergents10aDNA Primers10aDNA, Bacterial10aEndopeptidase K10aHumans10aleprosy10aMycobacterium leprae10aPathology, Molecular10apolymerase chain reaction10aRNA, Bacterial10aRNA, Ribosomal, 16S10aSkin10aSodium Dodecyl Sulfate1 aKamble R R1 aShinde V S1 aMadhale S P1 aKamble A A1 aRavikumar B P1 aJadhav R S00aExtraction and detection of Mycobacterium leprae DNA from ZNCF-stained skin smear slides for better identification of negative skin smears.  uhttp://medind.nic.in/iau/t10/i1/iaut10i1p57.htm  a57-90 v283 a<p>Abstract Identification of Mycobacterium leprae, which causes leprosy, is done by Ziehl Neelsen Carbol Fuchsin (ZNCF) stained slit skin smear microscopy that aids in the diagnosis and quantification of approximate bacterial load carried by the patient. We attempted M. leprae DNA extraction from 46 stained slit skin smear negative slides, using Proteinase K and SDS lysis, followed by ethanol precipitation. M. leprae specific primers (16SrRNA) were used for PCR-based amplification of DNA. We could detect M. leprae DNA in 15 (32.6%) samples. The method can be useful in the diagnosis of apparently slit skin smear negative leprosy cases.</p>  a1998-3646