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Abnormalities in intracellular processing and expression of interferon-gamma receptor in adherent cells from lepromatous leprosy patients.

Abstract

Peripheral blood mononuclear cells in lepromatous leprosy (LL) patients produce low levels of interferon-gamma (IFN-gamma) and interleukin-12 (IL-12), and these cells exhibit partial or complete deficiency in the IL-12 receptor. The behavior of the IFN-gamma receptor (IFN-gamma R) has not been described in cells from people with leprosy. We found higher levels of mRNA for IFN-gamma R1 and IFN-gamma R2 in adherent cells stimulated with IFN-gamma and Mycobacterium leprae membrane proteins from LL patients compared with healthy subjects. Flow cytometry showed no significant difference in IFN-gamma R1 expression between LL patients and healthy subjects. Immunoblotting detected only the mature glycosylated form of the 61-67 kDa IFN-gamma R2 protein in healthy subjects. In contrast, cells from LL patients showed three different expression patterns: (1) the immature deglycosylated form of the 34.8 kDa IFN-gamma R2 protein, (2) the mature glycosylated 61-67 kDa form, and (3) both forms. Our data indicate the existence of abnormalities in the intracellular processing and protein expression of the IFN-gamma R in response to specific stimuli such as IFN-gamma and M. leprae membrane proteins in adherent cells of LL patients.

More information

Type
Journal Article
Author
Guerrero-Velázquez C
Lopez-Roa RI
Delgado-Rizo V
Guillen-Vargas CM
Montoya-Buelna M
Fafutis-Morris M
Year of Publication
2010
Journal
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research
Volume
30
Issue
2
Number of Pages
99-105
Date Published
2010 Feb
Language
eng
ISSN Number
1557-7465
DOI
10.1089/jir.2008.0121
Alternate Journal
J. Interferon Cytokine Res.
Publication Language
eng

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