@article{8896,
  keywords = {Adult, Cell Adhesion, Female, Hela Cells, Humans, Leprosy, lepromatous, Leukocytes, Mononuclear, Male, Middle Aged, RNA, Messenger, Receptors, Interferon, Reverse Transcriptase Polymerase Chain Reaction},
  author = {Guerrero-Velázquez C and Lopez-Roa R and Delgado-Rizo V and Guillen-Vargas C and Montoya-Buelna M and Fafutis-Morris M},
  title = {Abnormalities in intracellular processing and expression of interferon-gamma receptor in adherent cells from lepromatous leprosy patients.},
  abstract = {<p>Peripheral blood mononuclear cells in lepromatous leprosy (LL) patients produce low levels of interferon-gamma (IFN-gamma) and interleukin-12 (IL-12), and these cells exhibit partial or complete deficiency in the IL-12 receptor. The behavior of the IFN-gamma receptor (IFN-gamma R) has not been described in cells from people with leprosy. We found higher levels of mRNA for IFN-gamma R1 and IFN-gamma R2 in adherent cells stimulated with IFN-gamma and Mycobacterium leprae membrane proteins from LL patients compared with healthy subjects. Flow cytometry showed no significant difference in IFN-gamma R1 expression between LL patients and healthy subjects. Immunoblotting detected only the mature glycosylated form of the 61-67 kDa IFN-gamma R2 protein in healthy subjects. In contrast, cells from LL patients showed three different expression patterns: (1) the immature deglycosylated form of the 34.8 kDa IFN-gamma R2 protein, (2) the mature glycosylated 61-67 kDa form, and (3) both forms. Our data indicate the existence of abnormalities in the intracellular processing and protein expression of the IFN-gamma R in response to specific stimuli such as IFN-gamma and M. leprae membrane proteins in adherent cells of LL patients.</p>},
  year = {2010},
  journal = {Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research},
  volume = {30},
  pages = {99-105},
  month = {2010 Feb},
  issn = {1557-7465},
  doi = {10.1089/jir.2008.0121},
  language = {eng},
}