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Whole-blood nested-PCR amplification of M. leprae-specific DNA for early diagnosis of leprosy.

Abstract

We evaluated the sensitivity and specificity of a nested-polymerase chain reaction (PCR) method for detection of Mycobacterium leprae DNA from whole blood. Whole-blood specimens were subjected to nested-PCR amplification of M. leprae repeat DNA sequences in 49 multibacillary (MB) and 30 paucibacillary (PB) leprosy patients, 96 household contacts (HHCs), 18 tuberculosis (TB) patients, and 35 normal healthy individuals. M. leprae DNA was detected in 95.92% (47/49) of MB, 70% (21/30) of PB, and 6.25% (6/96) of HHC, but it was not detected in 18 TB or 35 normal controls. The sensitivities of the anti-bovine serum albumin (ND-O-BSA) immunoglobulin M (IgM) and antifusion protein of ML0405-ML2331 IgG for MB were 97.96% and 89.8%, and these values for PB were 70% and 53.33%. However, the ND-O-BSA enzyme-linked immunosorbent assay (ELISA) had lower specificity, with relatively high false-positive results for TB patients (16.67%) and normal healthy controls (10%). Based on these promising findings, we propose the use of nested PCR of whole-blood samples along with ELISA test for early detection of leprosy cases.

More information

Type
Journal Article
Author
Wen Y
Xing Y
Yuan L
Liu J
Zhang Y
Li H
Year of Publication
2013
Journal
The American journal of tropical medicine and hygiene
Volume
88
Issue
5
Number of Pages
918-22
Date Published
2013 May
Language
eng
ISSN Number
1476-1645
DOI
10.4269/ajtmh.11-0253
Alternate Journal
Am. J. Trop. Med. Hyg.
Publication Language
eng