Whole-blood nested-PCR amplification of M. leprae-specific DNA for early diagnosis of leprosy.

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TitleWhole-blood nested-PCR amplification of M. leprae-specific DNA for early diagnosis of leprosy.
Publication TypeJournal Article
AuthorsWen Y, Xing Y, Yuan L-C, Liu J, Zhang Y, Li H-ying
Abbrev. JournalAm. J. Trop. Med. Hyg.
JournalThe American journal of tropical medicine and hygiene
Year of Publication2013
Volume88
Issue5
Pagination918-22
Publication Languageeng
KeywordsAnimals, Antibodies, Bacterial, Cattle, China, DNA, Bacterial, Early Diagnosis, Enzyme-Linked Immunosorbent Assay, Humans, Immunoglobulin M, Leprosy, Mycobacterium leprae, Polymerase Chain Reaction, Sensitivity and Specificity, Serum Albumin, Bovine
Abstract

We evaluated the sensitivity and specificity of a nested-polymerase chain reaction (PCR) method for detection of Mycobacterium leprae DNA from whole blood. Whole-blood specimens were subjected to nested-PCR amplification of M. leprae repeat DNA sequences in 49 multibacillary (MB) and 30 paucibacillary (PB) leprosy patients, 96 household contacts (HHCs), 18 tuberculosis (TB) patients, and 35 normal healthy individuals. M. leprae DNA was detected in 95.92% (47/49) of MB, 70% (21/30) of PB, and 6.25% (6/96) of HHC, but it was not detected in 18 TB or 35 normal controls. The sensitivities of the anti-bovine serum albumin (ND-O-BSA) immunoglobulin M (IgM) and antifusion protein of ML0405-ML2331 IgG for MB were 97.96% and 89.8%, and these values for PB were 70% and 53.33%. However, the ND-O-BSA enzyme-linked immunosorbent assay (ELISA) had lower specificity, with relatively high false-positive results for TB patients (16.67%) and normal healthy controls (10%). Based on these promising findings, we propose the use of nested PCR of whole-blood samples along with ELISA test for early detection of leprosy cases.

PubMed URL

http://www.ncbi.nlm.nih.gov/pubmed/23478578?dopt=Abstract

DOI10.4269/ajtmh.11-0253
PubMed Central IDPMC3752758