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Serum IgA Antibodies Specific to Antigens as Biomarkers for Leprosy Detection and Household Contact Tracking.

Abstract

Leprosy remains endemic in several developing countries, such as India and Brazil, in part due to delayed diagnosis that facilitates ongoing transmission. Although immunoglobulins against several antigens have been indicated for the early diagnosis, and IgA participates in the early stages of leprosy and in subclinical infection, relatively little research has examined anti-M. leprae IgA responses. Here, we investigated serum IgA reactivity against NDO-HSA, LID-1 and NDO-LID, in paucibacillary (PB) and multibacillary (MB) patients and their household contacts, using enzyme-linked immunosorbent assay (ELISA). Diagnostic accuracy of each ELISA was evaluated by receiver operating characteristic (ROC) curve analysis. Our data reveal elevated IgA serum levels against the three specific antigens in MB patients, whereas IgA reactivity in PB patients was increased only to NDO-HSA. Further, MB and PB household contacts displayed higher IgA reactivity to NDO-HSA than non-endemic controls. Our data suggest measurement of serum IgA against NDO-HSA as an additional tool in the diagnosis and classification of the disease, with potential utility for household contact follow-up.

More information

Type
Journal Article
Author
Silva K
de Oliveira E
Elias C
Pereira I
Pinheiro R
Sarno E
Duthie M
Teixeira H
Year of Publication
2021
Journal
Frontiers in medicine
Volume
8
Number of Pages
698495
Date Published
01/2021
Language
eng
ISSN Number
2296-858X
DOI
10.3389/fmed.2021.698495
Alternate Journal
Front Med (Lausanne)
PMID
34447763
Publication Language
eng