Screening for antimicrobial effect of probiotic organisms

Probiotic is a live microbial supplement which affects host’s health positively by improving its intestinal microbial balancethere by support the host health by interfering the immune system in different ways such as; producing cytokines, stimulating macrophages, increasing secretory IgA concentrations [1, 2, 3]. Antimicrobial activity is one of the most important selection criteria for probiotics. Antimicrobial activity targets the enteric pathogens. The present research aims to evaluate the antimicrobial effect of probiotic organism and to compare the safety therapeutic index over the commercially available antibiotics against the chosen clinical isolates. Total of 2 probiotic sources of fresh dairy (Curd) and non-dairy food (cucumber) products were collected from the market. Isolation and purification of lactic acid bacteria from dairy and non-dairy sample using De Man, Rogosa and Sharpe Agar (MRSA). Evaluation of probiotic potentials such as temperature sensitivity, lactose utilization, NaCl tolerance were also evaluated. Antimicrobial activity tested against the chosen clinical isolates such as Escherichia coli, Proteus sp, Klebsiella sp, Staphylococcus epidermidis, and Staphylococcus aureus. The present study focusing on characterizing the potential benefits of probiotic organism of dairy and non-dairy source. In the present study, all the selected LAB isolates were able to survive at temperature 25o, 30o, 37o and 40o. It was observed that the lactic acid bacterial isolates were able to tolerate 1-6.5% NaCl concentration. Antibiotic susceptibility pattern against selected clinical isolates was observed by using Kirby-Bauer disc diffusion method which showed significant antimicrobial activity of probiotic over the commercial available antibiotics. Study Concludes by stating that probiotic as a prophylactic and therapeutic choice of a alternative medicine.