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Reverse vaccinology and subtractive genomics approaches for identifying common therapeutics against Mycobacterium leprae and Mycobacterium lepromatosis.

Abstract

Background: and are gram-positive bacterial pathogens and the causative agents of leprosy in humans across the world. The elimination of leprosy cannot be achieved by multidrug therapy alone, and highlights the need for new tools and drugs to prevent the emergence of new resistant strains.

Methods: In this study, our contribution includes the prediction of vaccine targets and new putative drugs against leprosy, using reverse vaccinology and subtractive genomics. Six strains of and (4 and 2 strains, respectively) were used for comparison taking strain TN as the reference genome. Briefly, we used a combined reverse vaccinology and subtractive genomics approach.

Results: As a result, we identified 12 common putative antigenic proteins as vaccine targets and three common drug targets against and Furthermore the docking analysis using 28 natural compounds with three drug targets was done.

Conclusions: The bis-naphthoquinone compound Diospyrin (CID 308140) obtained from indigenous plant spp showed the most favored binding affinity against predicted drug targets, which can be a candidate therapeutic target in the future against leprosy.

More information

Type
Journal Article
Author
Jaiswal A
Tiwari S
Jamal S
Oliveira L
Sales-Campos H
Andrade-Silva L
Oliveira C
Ghosh P
Barh D
Azevedo V
Soares S
Rodrigues V
da Silva M
Year of Publication
2021
Journal
The journal of venomous animals and toxins including tropical diseases
Volume
27
Number of Pages
e20200027
Date Published
04/2021
Language
eng
ISSN Number
1678-9199
DOI
10.1590/1678-9199-JVATITD-2020-0027
Alternate Journal
J Venom Anim Toxins Incl Trop Dis
PMID
33889182
Publication Language
eng