Performance of recombinant K39 antigen in the diagnosis of Brazilian visceral leishmaniasis.

Printer-friendly version
TitlePerformance of recombinant K39 antigen in the diagnosis of Brazilian visceral leishmaniasis.
Publication TypeJournal Article
AuthorsCarvalho SGF, Lemos EM, Corey R, Dietze R
Abbrev. JournalAm. J. Trop. Med. Hyg.
JournalThe American journal of tropical medicine and hygiene
Year of Publication2003
Volume68
Issue3
Pagination321-4
Publication Languageeng
KeywordsAntigens, Protozoan, Case-Control Studies, Enzyme-Linked Immunosorbent Assay, Humans, Leishmaniasis, Visceral, Protozoan Proteins, Recombinant Proteins, Sensitivity and Specificity
Abstract

This study evaluated the performance of recombinant K39 (rK39) antigen in a immunochromatographic format (strip test) and a crude antigen enzyme-linked immunosorbent assay in the diagnosis of Brazilian visceral leishmaniasis (VL) in 128 consecutive patients with parasitologically proven infections (by microscopy and/or culture). For each patient, a medical history was obtained and a complete physical examination was performed. Controls included 10 healthy volunteers and 50 patients with other diseases: malaria (10), leprosy (9), Chagas' disease (10), tuberculosis (10), and cutaneous leishmaniasis (11). The sensitivities of the rK39 antigen strip test and the ELISA were 90% and 89%, respectively, while the specificities were 100% and 98%, respectively. Our study confirms the accuracy of the rK39 antigen strip test in the diagnosis of VL in a high prevalence population.

PubMed URL

http://www.ncbi.nlm.nih.gov/pubmed/12685638?dopt=Abstract

Link to full texthttp://www.ajtmh.org/docserver/fulltext/14761645/68/3/0680321.pdf?expires=1505398014&id=id&accname=guest&checksum=40CC2C54831C9BE75547BDA79834B4B9