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Human leukocyte antigen class I and class II alleles are associated with susceptibility and resistance in borderline leprosy patients from Southeast Brazil.

Abstract

BackgroundEvidence suggests that human leukocyte antigen (HLA) alleles influence the host immune response against Mycobacterium leprae. However, the association between HLA alleles and borderline (B) leprosy has not been studied. The aim of this study was to determine whether HLA class I and II molecules are associated with susceptibility or resistance to B leprosy including borderline-tuberculoid (BT), borderline-borderline (BB), and borderline-lepromatous (BL).MethodsDNA was obtained by the salting-out technique from the blood samples of 202 patients with B leprosy and 478 control subjects. HLA class I (A*, B*, and C* loci) and class II (DRB1* and DQB1* loci) genotypes were determined by polymerase chain reaction amplification and reverse hybridization with sequence-specific oligonucleotide probes and sequence-specific primers.ResultsThe case-controlled analysis results showed a significant association between B leprosy and HLA-C*05 (5.94% vs. 14.02%; p¿=¿0.002, OR¿=¿0.38, 95%CI¿=¿0.20¿0.73, pc¿=¿0.032) and HLA-DRB1*07 (16.34% vs. 26.77%; p¿=¿0.003, OR¿=¿0.53, 95%CI¿=¿0.3¿0.8, pc¿=¿0.039). A protective association was observed between BL leprosy and HLA-DQB1*02 (18.18% vs. 39.53%; p¿=¿0.005, OR¿=¿0.34, 95%CI¿=¿0.15¿0.75, pc¿=¿0.025). In reactional patients, a significant association was observed between HLA-B*15 (28.72% vs. 12.76%; p¿=¿0.011, OR¿=¿2.75, 95%CI¿=¿1.30¿5.85, pc¿=¿0.352) and predisposition to reversal reaction. Haplotype analysis showed that A*02-B*07-C*07-DRB1*15-DQB1*06 (2.97% vs. 1.04%; p¿=¿0.015) and A*02-B*40-C*03-DRB1*13-DQB1*06 (1.73% vs. 0.10%; p¿=¿0.0011) were associated with susceptibility to the B form. The presence of the HLA-DRB1*02 or HLA-DRB1*03/HLA-DQB1*01 haplotypes in B patients (22.05% vs. 33.0%; p¿=¿0.005) suggested the involvement of these haplotypes in this clinical form of the disease.ConclusionsThe results indicate the involvement of HLA class I and class II molecules in B leprosy and reversal reactions; it also suggest a role for HLA in polarization of the disease in this group of patients.

More information

Type
Journal Article
Author
Souza-Santana F
Marcos E
Nogueira M
Ura S
Tomimori J
Year of Publication
2015
Journal
BMC infectious diseases
Volume
15
Issue
1
Number of Pages
22
Language
eng
ISSN Number
1471-2334
Call Number
DE SOUZA SANTANA 2015
DOI
10.1186/s12879-015-0751-0
Alternate Journal
BMC Infect. Dis.
Publication Language
eng