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Histopathological examination of nerve samples from pure neural leprosy patients: obtaining maximum information to improve diagnostic efficiency.

Abstract

Nerve biopsy examination is an important auxiliary procedure for diagnosing pure neural leprosy (PNL). When acid-fast bacilli (AFB) are not detected in the nerve sample, the value of other nonspecific histological alterations should be considered along with pertinent clinical, electroneuromyographical and laboratory data (the detection of Mycobacterium leprae DNA with polymerase chain reaction and the detection of serum anti-phenolic glycolipid 1 antibodies) to support a possible or probable PNL diagnosis. Three hundred forty nerve samples [144 from PNL patients and 196 from patients with non-leprosy peripheral neuropathies (NLN)] were examined. Both AFB-negative and AFB-positive PNL samples had more frequent histopathological alterations (epithelioid granulomas, mononuclear infiltrates, fibrosis, perineurial and subperineurial oedema and decreased numbers of myelinated fibres) than the NLN group. Multivariate analysis revealed that independently, mononuclear infiltrate and perineurial fibrosis were more common in the PNL group and were able to correctly classify AFB-negative PNL samples. These results indicate that even in the absence of AFB, these histopathological nerve alterations may justify a PNL diagnosis when observed in conjunction with pertinent clinical, epidemiological and laboratory data.

More information

Type
Journal Article
Author
Antunes SLG
Chimelli L
Jardim MR
Vital RT
Nery JAC
Corte-Real S
Hacker MAVB
Sarno EN
Country
Brazil
Year of Publication
2012
Journal
Memorias do Instituto Oswaldo Cruz
Volume
107
Issue
2
Number of Pages
246-53
Date Published
2012 Mar
Language
eng
ISSN Number
1678-8060
DOI
10.1590/s0074-02762012000200015
Alternate Journal
Mem. Inst. Oswaldo Cruz
Publication Language
eng