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Evaluation of Leishmania species reactivity in human serologic diagnosis of leishmaniasis.

Abstract

The sensitivities and specificities of IgG-ELISA and IgG flow cytometry based techniques using different Leishmania species were determined using sera collected from 40 cutaneous or visceral leishmaniasis patients. The flow cytometry technique, using promastigote parasite forms, performed better than total soluble extract IgG-ELISA. At the species level, the use of Leishmania amazonensis and Leishmania major as antigens in enzyme linked immunosorbent assay (ELISA) decreased the overall sensitivity. To assess the specificity of these tests, sera from malaria, toxoplasmosis, amoebiasis, schistosomiasis, and leprosy patients were used. We also included sera from Leishmania non-infected endemic individuals. The cutaneous species displayed a decreased specificity in both assays. Although more sensitive, flow cytometry using promastigote parasite forms generally presented lower levels of specificity when compared with total extract of IgG-ELISA. Overall, the results of the study show the potential of IgG flow cytometry for the diagnosis of leishmaniasis. Although highly sensitive, a refinement of the flow cytometry method should be performed to improve the overall specificity.

More information

Type
Journal Article
Author
Silvestre R
Santarém N
Teixeira L
Cunha J
Schallig H
Cordeiro-da-Silva A
Year of Publication
2009
Journal
The American journal of tropical medicine and hygiene
Volume
81
Issue
2
Number of Pages
202-8
Date Published
2009 Aug
Language
eng
ISSN Number
1476-1645
Alternate Journal
Am. J. Trop. Med. Hyg.
Publication Language
eng

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