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Evaluation of Immunodiagnostic Tests for Leprosy in Brazil, China and Ethiopia.

Abstract

Leprosy remains persistently endemic in several low- or middle income countries. Transmission is still ongoing as indicated by the unabated rate of leprosy new case detection, illustrating the insufficiency of current prevention methods. Therefore, low-complexity tools suitable for large scale screening efforts to specifically detect M. leprae infection and diagnose disease are required. Previously, we showed that combined detection of cellular and humoral markers, using field-friendly lateral flow assays (LFAs), increased diagnostic potential for detecting leprosy in Bangladesh compared to antibody serology alone. In the current study we assessed the diagnostic performance of similar LFAs in three other geographical settings in Asia, Africa and South-America with different leprosy endemicity. Levels of anti-PGL-I IgM antibody (humoral immunity), IP-10, CCL4 and CRP (cellular immunity) were measured in blood collected from leprosy patients, household contacts and healthy controls from each area. Combined detection of these biomarkers significantly improved the diagnostic potential, particularly for paucibacillary leprosy in all three regions, in line with data obtained in Bangladesh. These data hold promise for the use of low-complexity, multibiomarker LFAs as universal tools for more accurate detection of M. leprae infection and different phenotypes of clinical leprosy.

More information

Type
Journal Article
Author
Hooij A
Tjon Kon Fat EM
Batista da Silva M
Carvalho Bouth R
Cunha Messias AC
Gobbo AR
Lema T
Bobosha K
Li J
Weng X
Salgado CG
Spencer JS
Corstjens PL A M
Geluk A
Year of Publication
2018
Journal
Scientific reports
Volume
8
Issue
1
Number of Pages
17920
Language
eng
ISSN Number
2045-2322
DOI
10.1038/s41598-018-36323-1
Alternate Journal
Sci Rep
Publication Language
eng