Engineered biomarkers for leprosy diagnosis using labeled and label-free analysis

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TitleEngineered biomarkers for leprosy diagnosis using labeled and label-free analysis
Publication TypeJournal Article
Authorsde Santana JF, da Silva MRB, Picheth GF, Yamanaka IB, Fogaça RL, Thomaz-Soccol V, Machado-de-Avila RA, Chávez-Olórtegui C, Sierakowski MR, de Freitas RA, Alvarenga LM, de Moura J
Abbrev. JournalTalanta
JournalTalanta
Year of Publication2018
Volume187
Publication Languageeng
KeywordsAg85B, Diagnosis, Engineered biomarkers, Immunosensor, Leprosy, Leprosy diagnosis, Mimotope, QCM, Synthetic peptide
Abstract

The biotechnological evolution towards the development of antigens to detect leprosy has been progressing. However, the identification of leprosy in paucibacillary patients, based solely on the antigen-antibody interaction still remains a challenge. The complexity of clinical manifestations requires innovative approaches to improve the sensitivity of assays to detect leprosy before the onset of symptoms, thus avoiding disabilities and contributing, indirectly, to reduce transmission. In this study, the strategies employed for early leprosy diagnosis were: i. using a phage-displayed mimotope (APDDPAWQNIFNLRR) which mimics an immunodominant sequence (PPNDPAWQRNDPILQ) of an antigen of Mycobacterium leprae known as Ag85B; ii. engineering the mimotope by adding a C-terminal flexible spacer (SGSG-C); iii. conjugating the mimotope to a carrier protein to provide better exposure to antibodies; iv. amplifying the signal using biotin–streptavidin detection system in an ELISA; and v. coating the optimized mimotope on a quartz crystal microbalance (QCM) sensor for label-free biosensing. The ELISA sensitivity increased up to 91.7% irrespective of the immunological profile of the 132 patients assayed. By using comparative modeling, the M. tuberculosis Ag85B was employed as a template to ascertain which features make the mimotope a good antigen in terms of its specificity. For the first time, a sensitive QCM-based immunosensor to detect anti M. leprae antibodies in human serum was used. M. leprae antibodies could also be detected in the sera of paucibacillary patients; thus, the use of a mimotope-derived synthetic peptide as bait for antibodies in a novel analytical label-free immunoassay for leprosy diagnosis exhibits great potential.

DOI10.1016/j.talanta.2018.05.027