Elevated Pentraxin-3 Concentrations in Patients With Leprosy: Potential Biomarker of Erythema Nodosum Leprosum.

Printer-friendly version
TitleElevated Pentraxin-3 Concentrations in Patients With Leprosy: Potential Biomarker of Erythema Nodosum Leprosum.
Publication TypeJournal Article
AuthorsMendes MA, de Carvalho DS, Amadeu TP, de Silva BJA, da Prata RBS, da Silva CO, Ferreira H, de Hacker MA, Nery JAC, Pinheiro RO, Sampaio EP, Sarno EN, Schmitz V
Abbrev. JournalJ. Infect. Dis.
JournalThe Journal of infectious diseases
Year of Publication2017
Volume216
Issue12
Pagination1635-1643
Publication Languageeng
Abstract

Background: Leprosy, the leading infectious cause of disability worldwide, remains a major public health challenge in the most severely affected countries despite the sharp decline in new cases in recent years. The search for biomarkers is essential to achieve a better understanding of the molecular and cellular mechanisms underlying the disease.

Methods: Pentraxin-3 (PTX3) analyses of sera from 87 leprosy patients with or without reactions were conducted via enzyme-linked immunosorbent assay. In situ identification of PTX3 in skin lesion was confirmed by quantitative reverse-transcription polymerase chain reaction, immunohistochemistry, and immunofluorescence assays.

Results: We found that PTX3 serum levels were higher in multibacillary patients when evaluated before the onset of acute erythema nodosum leprosum (ENL) and persistently elevated during reaction. Thalidomide treatment reduced PTX3 in the serum 7 days after starting treatment. In situ analyses have also demonstrated enhancement of PTX3 in ENL lesions and showed that treatment with thalidomide reduced its expression and the prominent neutrophilic infiltrate, a hallmark of the disease.

Conclusions: In summary, our study provides in vivo evidence that PTX3 is enhanced during ENL but not in reversal reaction and provides a new molecular target in ENL pathogenesis.

PubMed URL

http://www.ncbi.nlm.nih.gov/pubmed/29272525?dopt=Abstract

DOI10.1093/infdis/jix267