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Elevated Pentraxin-3 Concentrations in Patients With Leprosy: Potential Biomarker of Erythema Nodosum Leprosum.

Abstract

Background: Leprosy, the leading infectious cause of disability worldwide, remains a major public health challenge in the most severely affected countries despite the sharp decline in new cases in recent years. The search for biomarkers is essential to achieve a better understanding of the molecular and cellular mechanisms underlying the disease.

Methods: Pentraxin-3 (PTX3) analyses of sera from 87 leprosy patients with or without reactions were conducted via enzyme-linked immunosorbent assay. In situ identification of PTX3 in skin lesion was confirmed by quantitative reverse-transcription polymerase chain reaction, immunohistochemistry, and immunofluorescence assays.

Results: We found that PTX3 serum levels were higher in multibacillary patients when evaluated before the onset of acute erythema nodosum leprosum (ENL) and persistently elevated during reaction. Thalidomide treatment reduced PTX3 in the serum 7 days after starting treatment. In situ analyses have also demonstrated enhancement of PTX3 in ENL lesions and showed that treatment with thalidomide reduced its expression and the prominent neutrophilic infiltrate, a hallmark of the disease.

Conclusions: In summary, our study provides in vivo evidence that PTX3 is enhanced during ENL but not in reversal reaction and provides a new molecular target in ENL pathogenesis.

More information

Type
Journal Article
Author
Mendes MA
Carvalho DS
Amadeu TP
Silva BJA
Prata RBS
Silva CO
Ferreira H
Hacker MA
Nery JAC
Pinheiro RO
Sampaio EP
Sarno EN
Schmitz V
Year of Publication
2017
Journal
The Journal of infectious diseases
Volume
216
Issue
12
Number of Pages
1635-1643
Language
eng
ISSN Number
1537-6613
DOI
10.1093/infdis/jix267
Alternate Journal
J. Infect. Dis.
Publication Language
eng