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Detection of phenolic glycolipid I of Mycobacterium leprae in sera from leprosy patients before and after start of multidrug therapy.

Abstract

A total of 100 untreated new leprosy patients were recruited prospectively and examined for the presence of phenolic glycolipid I (PGL-I) antigen in their serum specimens by dot enzyme-linked immunosorbent assay (ELISA) using rabbit anti-PGL-I antiserum. The presence of circulating PGL-I antigen was closely related to the bacterial indices (BI) of the patients. The PGL-I antigen was detectable in 27 (93.1%) of 29 patients with a BI of 4.0 or above and in 15 (68.2%) of 22 patients with a BI of 3.0 to 3.9. However, none of the 37 patients with a BI of less than 1.9 had detectable PGL-I antigen by the methods used in this study. The level of PGL-I in serum declined rapidly by about 90% 1 month after the start of multidrug therapy. This study showed clearly that anti-PGL-I IgM antibodies and circulating PGL-I antigen levels reflect the bacterial loads in untreated leprosy patients. The serological parameters based on the PGL-I antigen may therefore be useful in the assessment of leprosy patients at the time of diagnosis and possibly in monitoring patients following chemotherapy.

More information

Type
Journal Article
Author
Cho S N
Cellona R V
Villahermosa L G
Fajardo T T
Balagon M V
Abalos R M
Tan E V
Walsh G P
Kim J D
Brennan P J
Year of Publication
2001
Journal
Clinical and diagnostic laboratory immunology
Volume
8
Issue
1
Number of Pages
138-42
Date Published
2001 Jan
Language
eng
ISSN Number
1071-412X
DOI
10.1128/CDLI.8.1.138-142.2001
Alternate Journal
Clin. Diagn. Lab. Immunol.
Publication Language
eng