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Clinicopathological Diagnosis of Leprosy: Comparative Evaluation of Three Staining Methods for Acid Fast bacilli in Slit Skin Smears and Biopsy Specimens
Abstract
Detection of acid fast bacilli (AFB) in slit smears and histopathological specimens is of paramount importance
for diagnosis and classification of leprosy. In this study the results of conventional staining technique for AFB
has been compared with modified rapid AFB and Fite Faraco stain in slit skin smears and punch biopsy
specimens from clinically diagnosed cases of leprosy. Processed skin biopsies and slit skin smears of 42
patients attending outdoor clinic of a tertiary care centre were stained with three stains viz Fite Faraco,
modified Rapid AFB and conventional Ziehl Neelsen staining. According to clinical diagnosis the maximum
number of patients belonged to Borderline Tuberculoid leprosy which correlated with histopathological
diagnosis of skin biopsy. Clinical and histopathological correlation was not observed in 14/42 cases -
histopathological categorization of biopsies from these cases revealed Indeterminate leprosy (9 cases),
Tuberculoid Leprosy (2 cases), Borderline Lepromatous (1 case), Histoid Leprosy (1 case) and Mid- Borderline
Leprosy (1 case). Maximum positivity for AFB was seen with Fite Faraco staining followed by modified Rapid
AFB both in the biopsy specimens and slit skin smears. Fite Faraco staining showed highest sensitivity in both
paucibacillary and multibacillary cases followed by modified rapid AFB and conventional AFB staining. Though
biopsy and slit skin smears have their individual diagnostic advantages and limitations, biopsy deserves to be
viewed as gold standard in case of difficulty in arriving at a confirmed diagnosis. Findings of this study need to
be validated in a larger number of leprosy cases at community level studies and correlated with classification
currently recommended by WHO and NLEP.
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Type
Journal Article