|Title||Influence of TNF and IL10 gene polymorphisms in the immunopathogenesis of leprosy in the south of Brazil.|
|Publication Type||Journal Article|
|Authors||Franceschi DSA, Mazini PS, Rudnick CCC, Sell AM, Tsuneto LT, Ribas ML, Peixoto PR, Visentainer JEL|
|Abbrev. Journal||Int. J. Infect. Dis.|
|Journal||International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases|
|Year of Publication||2009|
|Keywords||Brazil, Ethnic Groups, Female, Gene Frequency, Genotype, Humans, Interleukin-10, Leprosy, Male, Polymerase Chain Reaction, Polymorphism, Genetic, Polymorphism, Single Nucleotide, Reference Values, Tumor Necrosis Factor-alpha|
OBJECTIVE: To determine whether cytokine polymorphisms are associated with leprosy and/or their subtypes in a Brazilian population.
METHODS: Genotyping using polymerase chain reaction with sequence-specific primers (PCR-SSP) was performed for: TNF(-308/-238), IL2(-330/+166), IL6(-174), IFNG(+874), TGFB1(+869/+915), and IL10(-592/-819/-1082) in 240 healthy controls and 167 patients with leprosy.
RESULTS: For TNF(-308), a higher frequency of GG genotype (85.5% vs. 74.1% in healthy controls, p = 0.009), along with a decreased frequency of GA/AA genotypes was observed among leprosy patients as compared to the control group (14.5% vs. 25.9%, p = 0.009). The GG genotype was particularly higher in patients with tuberculoid (TT) and borderline (BB) leprosy (90.5% and 89.8%, respectively). Analysis of IL10 genotypes revealed a lower frequency of GCC/GCC haplotype in lepromatous leprosy (LL) patients (6.2%) in comparison to controls (15.4%).
CONCLUSION: It is suggested that the G-->A substitution at position -308 in the TNF promoter region plays an important role in leprosy patients.