Genotyping of Mycobacterium leprae from Brazilian leprosy patients suggests the occurrence of reinfection or of bacterial population shift during disease relapse.

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TitleGenotyping of Mycobacterium leprae from Brazilian leprosy patients suggests the occurrence of reinfection or of bacterial population shift during disease relapse.
Publication TypeJournal Article
Authorsda Silva Rocha A, Cunha Dos Santos AA, Pignataro P, Nery JA, de Miranda AB, Soares DF, Brum Fontes AN, Miranda A, Ferreira H, Boéchat N, Novisck Gallo ME, Sarno EN, De Oliveira MLW, Suffys PN
Abbrev. JournalJ. Med. Microbiol.
JournalJournal of medical microbiology
Place Publisheds.l.
PublisherSociety for General Microbiology
Year of Publication2011
Volume60
IssuePt 10
Pagination1441-6
Publication Languageeng
KeywordsAdult, Aged, Bacterial Proteins, Biopsy, Brazil, Female, Genotype, Humans, Leprosy, Male, Middle Aged, Molecular Typing, Mycobacterium leprae, Recurrence, Repetitive Sequences, Nucleic Acid, Sequence Analysis, DNA, Skin
Abstract

We performed genotyping of Mycobacterium leprae present in skin biopsy samples that were collected during the first and the second disease occurrences from eight leprosy patients, seven of whom were diagnosed as suffering from disease relapse. Sequence analysis of part of the M. leprae rpoB, folP1, gyrB and gyrA genes did not show genetic change that supported the presence of drug-resistant bacilli. However, we observed a synonymous nucleotide change at position 297 of gyrA among five of these patients, one presenting C to T (CgyrAT) and four presenting T to C (TgyrAC) at this position. Additional genotyping by analysis of the four short tandem repeats GAA, GTA9, AT17 and TA18 showed that the gyrA single nucleotide polymorphism change was accompanied by a change in short tandem repeat genotype. Our data suggest that leprosy relapse in these patients, living in an area endemic for leprosy, could be caused by M. leprae with a genotype different from the one that caused initial disease.

PubMed URL

http://www.ncbi.nlm.nih.gov/pubmed/21596907?dopt=Abstract

DOI10.1099/jmm.0.029389-0
Link to full texthttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3347867/pdf/1441.pdf
PubMed Central IDPMC3347867
Grant ListR01 AI047197 / AI / NIAID NIH HHS / United States