Genotyping comparison of Mycobacterium leprae isolates by VNTR analysis from nasal samples in a Brazilian endemic region.

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TitleGenotyping comparison of Mycobacterium leprae isolates by VNTR analysis from nasal samples in a Brazilian endemic region.
Publication TypeJournal Article
AuthorsLima LNC, Frota CC, Suffys PN, Fontes ANB, Mota RMS, Almeida RLF, de Andrade Pontes MA, de Gonçalves HSá, Kendall C, Kerr LRS
Abbrev. JournalPathog Glob Health
JournalPathogens and global health
Year of Publication2018
Pagination1-7
Publication Languageeng
KeywordsBrazil, Cluster Analysis, Genetic marker, Genotype, Leprosy, Mycobacterium leprae, Nasal Cavity, VNTR loci
Abstract

This study analyzed the genetic diversity by MIRU-VNTR of Mycobacterium leprae isolates from nasal cavities and related to epidemiological and clinical data. The sample consisted of 48 newly diagnosed leprosy cases that tested positive for M. leprae PCR in nasal secretion (NS) attending to the National Reference Center of Dermatology Dona Libania (CDERM), Fortaleza, Brazil. Total DNA was extracted from NS of each patient and used for amplification of four M. leprae VNTR loci. Four clusters of M. leprae isolates were formed with identical genotypes. In the spatial analysis, 12 leprosy cases presented similar genotypes organized into 4 clusters. The most common genotypes in the current study was AC8b: 8, AC9: 7, AC8a: 8, GTA9: 10, which may represent a genotype of circulating strains most often in Ceará. A minimum set of four MIRU-VNTR loci was demonstrated to study the genetic diversity of M. leprae isolates from NS.

PubMed URL

http://www.ncbi.nlm.nih.gov/pubmed/29405082?dopt=Abstract

DOI10.1080/20477724.2018.1427308