Diagnostic challenges of single plaque-like lesion paucibacillary leprosy.

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TitleDiagnostic challenges of single plaque-like lesion paucibacillary leprosy.
Publication TypeJournal Article
AuthorsBarbieri RR, Sales AM, Illarramendi X, Moraes MO, da Nery JAC, Moreira SJM, Sarno EN, de Machado AM, Bozza FA
Abbrev. JournalMem. Inst. Oswaldo Cruz
JournalMemorias do Instituto Oswaldo Cruz
Year of Publication2014
Publication Languageeng
KeywordsBrazil, Diagnosis, Paucibacillary leprosy, Polymerase Chain Reaction
Abstract

The diagnosis of single-lesion paucibacillary leprosy remains a challenge. Reviews by expert dermatopathologists and quantitative polymerase chain reaction (qPCR) results obtained from 66 single-plaque biopsy samples were compared. Histological findings were graded as high (HP), medium (MP) or low (LP) probability of leprosy or other dermatopathy (OD). Mycobacterium leprae-specific genes were detected using qPCR. The biopsies of 47 out of 57 clinically diagnosed patients who received multidrug therapy were classified as HP/MP, eight of which were qPCR negative. In the LP/OD (n = 19), two out of eight untreated patients showed positive qPCR results. In the absence of typical histopathological features, qPCR may be utilised to aid in final patient diagnosis, thus reducing overtreatment and delay in diagnosis.

PubMed URL

http://www.ncbi.nlm.nih.gov/pubmed/25338157?dopt=Abstract

Link to full texthttp://www.scielo.br/pdf/mioc/2014nahead/0074-0276-mioc-0074-0276140212.pdf