TY - JOUR
KW - Active Transport, Cell Nucleus
KW - Animals
KW - Apoptosis
KW - Apoptosis Inducing Factor
KW - Bacterial Vaccines
KW - Caspase 3
KW - Cell Nucleus
KW - Culture Media, Conditioned
KW - Cytochromes c
KW - Down-Regulation
KW - Female
KW - Injections
KW - Lipopolysaccharides
KW - Macrophages, Peritoneal
KW - Male
KW - Mice
KW - Mice, Inbred BALB C
KW - Mitochondria
KW - Mycobacterium
KW - Poly (ADP-Ribose) Polymerase-1
KW - Poly(ADP-ribose) Polymerases
KW - Protein Kinase C-delta
KW - Protein Kinase Inhibitors
KW - Proteolysis
KW - Proteomics
KW - Bcl-2-Associated X Protein
AU - Pandey RK
AU - Bhatt K
AU - Dahiya Y
AU - Sodhi A
AB - <p>Mycobacterium indicus pranii (MIP), also known as Mw, is a saprophytic, non-pathogenic strain of Mycobacterium and is commercially available as a heat-killed vaccine for leprosy and recently tuberculosis (TB) as part of MDT. In this study we provide evidence that cell-free supernatant collected from original MIP suspension induces rapid and enhanced apoptosis in mouse peritoneal macrophages in vitro. It is demonstrated that the MIP cell-free supernatant induced apoptosis is mitochondria-mediated and caspase independent and involves mitochondrial translocation of Bax and subsequent release of AIF and cytochrome c from the mitochondria. Experiments with pharmacological inhibitors suggest a possible role of PKC in mitochondria-mediated apoptosis of macrophages.</p>
BT - PloS one
C1 - http://www.ncbi.nlm.nih.gov/pubmed/21347304?dopt=Abstract
DA - 2011 Feb 11
DO - 10.1371/journal.pone.0017093
IS - 2
J2 - PLoS ONE
LA - eng
N2 - <p>Mycobacterium indicus pranii (MIP), also known as Mw, is a saprophytic, non-pathogenic strain of Mycobacterium and is commercially available as a heat-killed vaccine for leprosy and recently tuberculosis (TB) as part of MDT. In this study we provide evidence that cell-free supernatant collected from original MIP suspension induces rapid and enhanced apoptosis in mouse peritoneal macrophages in vitro. It is demonstrated that the MIP cell-free supernatant induced apoptosis is mitochondria-mediated and caspase independent and involves mitochondrial translocation of Bax and subsequent release of AIF and cytochrome c from the mitochondria. Experiments with pharmacological inhibitors suggest a possible role of PKC in mitochondria-mediated apoptosis of macrophages.</p>
PY - 2011
EP - e17093
T2 - PloS one
TI - Mycobacterium indicus pranii supernatant induces apoptotic cell death in mouse peritoneal macrophages in vitro.
UR - http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0017093
VL - 6
SN - 1932-6203
ER -