02015nas a2200313   4500000000100000008004100001260001300042653001200055653002600067653003800093653001900131653001100150653002100161653001500182653001800197653003200215653002400247100001600271700001600287700001500303700001200318700002000330700002400350245009500374300001000469490000700479520120100486022001401687       2009                            d  c2009 Aug10aAnimals10aAntibodies, Protozoan10aEnzyme-Linked Immunosorbent Assay10aFlow Cytometry10aHumans10aImmunoglobulin G10aLeishmania10aLeishmaniasis10aSensitivity and Specificity10aSpecies Specificity1 aSilvestre R1 aSantarém N1 aTeixeira L1 aCunha J1 aSchallig HD F H1 aCordeiro-da-Silva A00aEvaluation of Leishmania species reactivity in human serologic diagnosis of leishmaniasis.  a202-80 v813 a<p>The sensitivities and specificities of IgG-ELISA and IgG flow cytometry based techniques using different Leishmania species were determined using sera collected from 40 cutaneous or visceral leishmaniasis patients. The flow cytometry technique, using promastigote parasite forms, performed better than total soluble extract IgG-ELISA. At the species level, the use of Leishmania amazonensis and Leishmania major as antigens in enzyme linked immunosorbent assay (ELISA) decreased the overall sensitivity. To assess the specificity of these tests, sera from malaria, toxoplasmosis, amoebiasis, schistosomiasis, and leprosy patients were used. We also included sera from Leishmania non-infected endemic individuals. The cutaneous species displayed a decreased specificity in both assays. Although more sensitive, flow cytometry using promastigote parasite forms generally presented lower levels of specificity when compared with total extract of IgG-ELISA. Overall, the results of the study show the potential of IgG flow cytometry for the diagnosis of leishmaniasis. Although highly sensitive, a refinement of the flow cytometry method should be performed to improve the overall specificity.</p>  a1476-1645