01778nas a2200325   4500000000100000008004100001260001300042653002700055653002400082653002300106653002000129653001300149653001100162653001600173653001900189653001200208653002100220653001800241653002500259653003100284100001200315700001600327700001400343700001500357245013600372300001100508490000800519520091100527022001401438       1990                            d  c1990 Aug10aAntibodies, Monoclonal10aAntigens, Bacterial10aBacterial Vaccines10aCross Reactions10aEpitopes10aHumans10aImmune Sera10aImmunoblotting10aleprosy10aMolecular Weight10aMycobacterium10aMycobacterium leprae10aMycobacterium tuberculosis1 aGanju L1 aMukherjee R1 aBatra H V1 aTalwar G P00aImmuno-blot analysis of antigens of Mycobacterium w: a candidate anti-leprosy vaccine using monoclonal antibodies and patient sera.  a378-850 v2733 a<p>The presence of determinants immunologically cross-reactive with M. leprae and M. tuberculosis in Mycobacterium w (M. w) has been revealed by immunoblotting using cross-reactive and specific monoclonal antibodies (Moabs) to M. leprae and M. tuberculosis. Three of the seven M. leprae and one of the two M. tuberculosis "specific" Moabs showed reactivity with M. w antigens. Reactions were also manifest with cross-reactive Moabs. One out of the three Moabs raised to M. leprae and three of six to M. tuberculosis demonstrated reactivity with M. w antigens. Extensive reactivity of M. w antigens was also observed with patient sera; sera from leprosy patients reacted prominently with M. w antigens at 14-17 KDa and sera of active tuberculosis patients exhibited reactivity at 21 KDa antigens of M. w. Four out of 30 healthy individuals living in an endemic area showed reactivity with M. w antigens.</p>  a0934-8840