02482nas a2200361   4500000000100000008004100001260001300042653001200055653002600067653002400093653002500117653002000142653001600162653001100178653002100189653001200210653000900222653002400231653001800255653002500273100001600298700001400314700001700328700001900345700001500364700001500379245016000394856009000554300001000644490000700654520144500661022001402106       1989                            d  c1989 Nov10aAnimals10aAntibodies, Bacterial10aAntigens, Bacterial10aCase-Control Studies10aCross Reactions10aGlycolipids10aHumans10aImmunoglobulin M10aleprosy10aMice10aMice, Inbred BALB C10aMycobacterium10aMycobacterium leprae1 aMoudgil K D1 aGupta S K1 aNaraynan P R1 aSrivastava L M1 aMishra R S1 aTalwar G P00aAntibody response to phenolic glycolipid I and Mycobacterium w antigens and its relation to bacterial load in M. leprae-infected mice and leprosy patients.  uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1534668/pdf/clinexpimmunol00080-0074.pdf  a214-80 v783 a<p>Twenty-six inbred BALB/cBy mice were infected with live Mycobacterium leprae by injecting 6 x 10(3) bacilli in the hind footpad. Bleeds were collected at monthly intervals. After 6 months, acid-fast bacilli (AFB) were harvested monthly from the footpad of mice. The sera were analysed in enzyme immunoassay for antibodies against phenolic glycolipid I (PGL-I) of M. leprae and antigens of Mycobacterium w (M. w); 21 out of 26 (80.7%) mice demonstrated the presence of antibodies against PGL-I and M. w . Anti-M. w antibodies appeared slightly earlier than did anti-PGL-I antibodies. The titre of anti-M. w antibodies was higher than that of anti-PGL-I antibodies. The mice giving a positive antibody response had more than 7 x 10(5) AFB/footpad. The coefficient of correlation (r) between the number of AFB and antibody titres at the time of harvest was 0.566 for PGL-I and 0.628 for M. w. The value of r for bacterial index and antibody titres in 188 leprosy patients was 0.510 for PGL-I and 0.418 for M. w; these values were statistically significant (P < 0.001). The decrease in bacterial index and antibody titres in treated lepromatous leprosy patients correlated with increase in the duration of chemotherapy. The measurement of anti-PGL-I antibodies of IgM class may serve as an adjunct to skin biopsy and skin-slit smear for serial monitoring of the bacterial load in the course of chemotherapy in leprosy control programmes.</p>  a0009-9104