02419nas a2200337   4500000000100000008004100001653001600042653003400058653002700092653001600119653003100135653002400166653003200190653001400222653000900236653001100245653001100256653001400267653002000281653001400301653001000315100001200325700001500337700001000352245013800362856005900500300001100559490000700570520149000577022001402067       2012                            d10aYoung Adult10aT-Lymphocytes, Helper-Inducer10aReceptors, Immunologic10aNeutrophils10aMycobacterium tuberculosis10aMycobacterium bovis10aMycobacterium avium Complex10aMonocytes10aMale10aHumans10aFemale10aCytokines10aCells, Cultured10aApoptosis10aAdult1 aHilda N1 aSelvaraj A1 aDas S00aMycobacterium tuberculosis H37Rv is more effective compared to vaccine strains in modulating neutrophil functions: an in vitro study.  uhttp://femsim.oxfordjournals.org/content/66/3/372.long  a372-810 v663 a<p>Neutrophils are the primary cells contributing to initial defense against mycobacteria. Yet, little is known about the potential of various mycobacterial strains to stimulate neutrophils. This study was focused to compare the differential capacity of vaccine strains, Mycobacterium bovis bacillus Calmette-Guerin (BCG) and Mycobacterium indicus pranii (Mw), and laboratory strain H37Rv to activate and enhance neutrophil functions. The expression of phenotypic markers like Fcγ receptor, toll-like receptor (TLR), and chemokine receptor; secretion of pro-inflammatory cytokines; and the rate of apoptosis were studied in infected neutrophils. Increased expression of CD32, CD64, TLR4, and CXCR3; increased TNF-α secretion; and downregulation of early apoptosis were observed in H37Rv-infected neutrophils. Among the vaccine strains, BCG increased the expression of only CD32 on neutrophils, while Mw was comparatively ineffective. To understand the paracrine role of neutrophils, the supernatants from infected neutrophils were used to stimulate monocytes and T helper cells. The secretory molecules from all infected neutrophils increased the expression of CCR5 on monocytes, whereas only H37Rv-infected supernatant increased the expression of CCR7 on monocytes and CD69 on T cells. Thus, H37Rv was more effective in activating neutrophils and in turn stimulating monocytes and T cells. By comparison, vaccine strains were less effective in modulating neutrophil functions.</p>
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