02165nas a2200493   4500000000100000008004100001260001600042653003500058653001200093653001400105653003000119653002300149653001400172653001700186653003100203653001800234653002000252653001100272653001500283653002400298653002800322653000900350653000900359653002400368653001700392653001800409653003500427653003300462653002700495653003000522653001600552653001500568653003100583100001400614700001200628700001300640700001200653245011600665856007900781300001100860490000600871520078000877022001401657       2011                            d  c2011 Feb 1110aActive Transport, Cell Nucleus10aAnimals10aApoptosis10aApoptosis Inducing Factor10aBacterial Vaccines10aCaspase 310aCell Nucleus10aCulture Media, Conditioned10aCytochromes c10aDown-Regulation10aFemale10aInjections10aLipopolysaccharides10aMacrophages, Peritoneal10aMale10aMice10aMice, Inbred BALB C10aMitochondria10aMycobacterium10aPoly (ADP-Ribose) Polymerase-110aPoly(ADP-ribose) Polymerases10aProtein Kinase C-delta10aProtein Kinase Inhibitors10aProteolysis10aProteomics10aBcl-2-Associated X Protein1 aPandey RK1 aBhatt K1 aDahiya Y1 aSodhi A00aMycobacterium indicus pranii supernatant induces apoptotic cell death in mouse peritoneal macrophages in vitro.  uhttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0017093  ae170930 v63 a<p>Mycobacterium indicus pranii (MIP), also known as Mw, is a saprophytic, non-pathogenic strain of Mycobacterium and is commercially available as a heat-killed vaccine for leprosy and recently tuberculosis (TB) as part of MDT. In this study we provide evidence that cell-free supernatant collected from original MIP suspension induces rapid and enhanced apoptosis in mouse peritoneal macrophages in vitro. It is demonstrated that the MIP cell-free supernatant induced apoptosis is mitochondria-mediated and caspase independent and involves mitochondrial translocation of Bax and subsequent release of AIF and cytochrome c from the mitochondria. Experiments with pharmacological inhibitors suggest a possible role of PKC in mitochondria-mediated apoptosis of macrophages.</p>  a1932-6203