02014nas a2200325   4500000000100000008004100001260001300042653002400055653002400079653002300103653001800126653001600144653001900160653001100179653002800190653001800218653003100236653003000267653002400297100001400321700001300335700001800348700001500366245010600381856004100487300001100528490000700539520112800546022001401674       1994                            d  c1994 Jun10aAmino Acid Sequence10aAntigens, Bacterial10aBacterial Vaccines10aBase Sequence10aDNA Primers10aDNA, Bacterial10aHumans10aMolecular Sequence Data10aMycobacterium10aMycobacterium tuberculosis10apolymerase chain reaction10aSpecies Specificity1 aReddi P P1 aAmin A G1 aKhandekar P S1 aTalwar G P00aMolecular definition of unique species status of Mycobacterium w; a candidate leprosy vaccine strain.  uhttp://ila.ilsl.br/pdfs/v62n2a05.pdf  a229-360 v623 a<p>Mycobacterium w, a candidate leprosy vaccine strain, is an atypical cultivable mycobacterium. Based on its growth and metabolic properties, M. w was listed in Runyon Group IV, along with other rapid growers such as M. fortuitum, M. smegmatis, M. chelonae and M. vaccae. However, M. w was not fully identical to any one of these. In the present study, a molecular biology approach was used to define the species identity of M. w in a manner that allows reliable comparison to be made with over 30 known mycobacterial species. A 383-bp region, present at the amino terminus of the conserved mycobacterial 65-kDa gene, has been polymerase chain reaction (PCR) amplified in M. w and DNA sequence was determined. A comparison of the M. w DNA sequence with those of M. tuberculosis, M. avium, M. paratuberculosis and M. fortuitum revealed a species-specific polymorphism, i.e., the presence of nucleotide substitutions unique to M. w. In an alternate approach, a 441-bp region, also a part of the 65-kDa gene, has been PCR amplified in M. w and a Hae III restriction pattern was generated.(ABSTRACT TRUNCATED AT 250 WORDS)</p>  a0148-916X