@article{21650,
  keywords = {Active Transport, Cell Nucleus, Animals, Apoptosis, Apoptosis Inducing Factor, Bacterial Vaccines, Caspase 3, Cell Nucleus, Culture Media, Conditioned, Cytochromes c, Down-Regulation, Female, Injections, Lipopolysaccharides, Macrophages, Peritoneal, Male, Mice, Mice, Inbred BALB C, Mitochondria, Mycobacterium, Poly (ADP-Ribose) Polymerase-1, Poly(ADP-ribose) Polymerases, Protein Kinase C-delta, Protein Kinase Inhibitors, Proteolysis, Proteomics, Bcl-2-Associated X Protein},
  author = {Pandey RK and Bhatt K and Dahiya Y and Sodhi A},
  title = {Mycobacterium indicus pranii supernatant induces apoptotic cell death in mouse peritoneal macrophages in vitro.},
  abstract = {<p>Mycobacterium indicus pranii (MIP), also known as Mw, is a saprophytic, non-pathogenic strain of Mycobacterium and is commercially available as a heat-killed vaccine for leprosy and recently tuberculosis (TB) as part of MDT. In this study we provide evidence that cell-free supernatant collected from original MIP suspension induces rapid and enhanced apoptosis in mouse peritoneal macrophages in vitro. It is demonstrated that the MIP cell-free supernatant induced apoptosis is mitochondria-mediated and caspase independent and involves mitochondrial translocation of Bax and subsequent release of AIF and cytochrome c from the mitochondria. Experiments with pharmacological inhibitors suggest a possible role of PKC in mitochondria-mediated apoptosis of macrophages.</p>},
  year = {2011},
  journal = {PloS one},
  volume = {6},
  pages = {e17093},
  month = {2011 Feb 11},
  issn = {1932-6203},
  url = {http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0017093},
  doi = {10.1371/journal.pone.0017093},
  language = {eng},
}