@article{18074,
  keywords = {Amino Acid Sequence, Antigens, Bacterial, Bacterial Vaccines, Base Sequence, DNA Primers, DNA, Bacterial, Humans, Molecular Sequence Data, Mycobacterium, Mycobacterium tuberculosis, polymerase chain reaction, Species Specificity},
  author = {Reddi P P and Amin A G and Khandekar P S and Talwar G P},
  title = {Molecular definition of unique species status of Mycobacterium w; a candidate leprosy vaccine strain.},
  abstract = {<p>Mycobacterium w, a candidate leprosy vaccine strain, is an atypical cultivable mycobacterium. Based on its growth and metabolic properties, M. w was listed in Runyon Group IV, along with other rapid growers such as M. fortuitum, M. smegmatis, M. chelonae and M. vaccae. However, M. w was not fully identical to any one of these. In the present study, a molecular biology approach was used to define the species identity of M. w in a manner that allows reliable comparison to be made with over 30 known mycobacterial species. A 383-bp region, present at the amino terminus of the conserved mycobacterial 65-kDa gene, has been polymerase chain reaction (PCR) amplified in M. w and DNA sequence was determined. A comparison of the M. w DNA sequence with those of M. tuberculosis, M. avium, M. paratuberculosis and M. fortuitum revealed a species-specific polymorphism, i.e., the presence of nucleotide substitutions unique to M. w. In an alternate approach, a 441-bp region, also a part of the 65-kDa gene, has been PCR amplified in M. w and a Hae III restriction pattern was generated.(ABSTRACT TRUNCATED AT 250 WORDS)</p>},
  year = {1994},
  journal = {International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association},
  volume = {62},
  pages = {229-36},
  month = {1994 Jun},
  issn = {0148-916X},
  url = {http://ila.ilsl.br/pdfs/v62n2a05.pdf},
  language = {eng},
}