TY - JOUR
KW - Adenosine Triphosphate
KW - Animals
KW - Dose-Response Relationship, Drug
KW - Humans
KW - Leprostatic Agents
KW - leprosy
KW - Microbial Sensitivity Tests
KW - Mycobacterium leprae
KW - Photometry
AU - Katoch V M
AU - Katoch K
AU - Shivannavar C T
AU - Sharma V D
AU - Patil M A
AU - Bharadwaj V P
AB - <p>In this study, the ATP content of M. leprae exposed to various antimicrobial agents has been measured to evaluate its usefulness in drug sensitivity screening. Purified M. leprae suspensions from human biopsies have been incubated at 30 degrees C in a modified Dubos medium in the presence of different concentrations of various drugs viz., Rifampicin, Ethionamide, Ethambutol, Cycloserine, Dapsone, Clofazimine, Erythromycin and Tetracycline. ATP levels were estimated at 0, 7 days, 14 days of incubation by the procedures modified and standardised at this laboratory. ATP decay was accelerated by ethionamide, rifampicin, clofazimine, dapsone, erythromycin and to a lesser extent by cycloserine, whereas ethambutol and tetracycline did not have any significant effect. The rate of decay depended on the concentrations of these drugs. ATP assay promises to be a useful system for in vitro drug sensitivity screening against M. leprae isolated from patients.</p>
BT - Indian journal of leprosy
C1 - http://www.ncbi.nlm.nih.gov/pubmed/2671179?dopt=Abstract
CN - Infolep Library - available
DA - 1989 Jul
IS - 3
J2 - Indian J Lepr
LA - eng
N2 - <p>In this study, the ATP content of M. leprae exposed to various antimicrobial agents has been measured to evaluate its usefulness in drug sensitivity screening. Purified M. leprae suspensions from human biopsies have been incubated at 30 degrees C in a modified Dubos medium in the presence of different concentrations of various drugs viz., Rifampicin, Ethionamide, Ethambutol, Cycloserine, Dapsone, Clofazimine, Erythromycin and Tetracycline. ATP levels were estimated at 0, 7 days, 14 days of incubation by the procedures modified and standardised at this laboratory. ATP decay was accelerated by ethionamide, rifampicin, clofazimine, dapsone, erythromycin and to a lesser extent by cycloserine, whereas ethambutol and tetracycline did not have any significant effect. The rate of decay depended on the concentrations of these drugs. ATP assay promises to be a useful system for in vitro drug sensitivity screening against M. leprae isolated from patients.</p>
PY - 1989
SP - 333
EP - 44
T2 - Indian journal of leprosy
TI - Application of ATP assay for in vitro drug screening testing against human derived M. leprae.
VL - 61
SN - 0254-9395
ER -