TY - JOUR
KW - Antigens, Bacterial
KW - BCG Vaccine
KW - Cell Line
KW - Humans
KW - leprosy
KW - Lymphocyte Activation
KW - Mycobacterium leprae
KW - Reproducibility of Results
KW - T-Lymphocytes
AU - Lee S P
AU - Stoker N G
AU - Grant K A
AU - Handzel Z T
AU - Hussain R
AU - McAdam K P
AU - Dockrell H M
AB - <p>Antigens of armadillo-derived Mycobacterium leprae sonic extract were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and blotted onto a nitrocellulose membrane, and the unstained blot was converted into 20 fractions of antigen-bearing particles. These were tested in cellular proliferation assays, and reproducible results were obtained between batches of fractions. Peripheral blood mononuclear cells from healthy contacts of leprosy patients (presumed to have protective immunity) were tested with the fractions to investigate which antigens they recognized. A small group of tuberculoid leprosy patients were also tested. Both groups showed a wide range of responses. Almost every fraction stimulated proliferation with at least one donor, yet none was clearly immunodominant or inhibitory in either group. Thus, protective immunity did not appear to be associated with proliferation caused by any single fraction.</p>
BT - Infection and immunity
C1 - http://www.ncbi.nlm.nih.gov/pubmed/2663725?dopt=Abstract
DA - 1989 Aug
IS - 8
J2 - Infect. Immun.
LA - eng
N2 - <p>Antigens of armadillo-derived Mycobacterium leprae sonic extract were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and blotted onto a nitrocellulose membrane, and the unstained blot was converted into 20 fractions of antigen-bearing particles. These were tested in cellular proliferation assays, and reproducible results were obtained between batches of fractions. Peripheral blood mononuclear cells from healthy contacts of leprosy patients (presumed to have protective immunity) were tested with the fractions to investigate which antigens they recognized. A small group of tuberculoid leprosy patients were also tested. Both groups showed a wide range of responses. Almost every fraction stimulated proliferation with at least one donor, yet none was clearly immunodominant or inhibitory in either group. Thus, protective immunity did not appear to be associated with proliferation caused by any single fraction.</p>
PY - 1989
SP - 2475
EP - 80
T2 - Infection and immunity
TI - Cellular immune responses of leprosy contacts to fractionated Mycobacterium leprae antigens.
VL - 57
SN - 0019-9567
ER -