TY - JOUR KW - Animals KW - Biopsy KW - Blotting, Southern KW - DNA, Bacterial KW - Electrophoresis, Agar Gel KW - Gene Amplification KW - Humans KW - Kinetics KW - leprosy KW - Mycobacterium leprae KW - polymerase chain reaction KW - Repetitive Sequences, Nucleic Acid AU - Woods S A AU - Cole S T AB -
A simple procedure based on the polymerase chain reaction has been developed to detect Mycobacterium leprae, rapidly and unambiguously, in biological samples. Its application to small numbers of M. leprae cells (approximately 10(2] isolated from armadillo liver, mouse footpads or human biopsies is discussed.
BT - FEMS microbiology letters C1 - http://www.ncbi.nlm.nih.gov/pubmed/2693204?dopt=Abstract DA - 1989 Dec DO - 10.1016/0378-1097(89)90235-8 IS - 3 J2 - FEMS Microbiol. Lett. LA - eng N2 -A simple procedure based on the polymerase chain reaction has been developed to detect Mycobacterium leprae, rapidly and unambiguously, in biological samples. Its application to small numbers of M. leprae cells (approximately 10(2] isolated from armadillo liver, mouse footpads or human biopsies is discussed.
PY - 1989 SP - 305 EP - 9 T2 - FEMS microbiology letters TI - A rapid method for the detection of potentially viable Mycobacterium leprae in human biopsies: a novel application of PCR. VL - 53 SN - 0378-1097 ER -