TY - JOUR
KW - Amino Acid Sequence
KW - Antigens, Bacterial
KW - BCG Vaccine
KW - Carrier State
KW - Cell Line
KW - Cell Separation
KW - Female
KW - Humans
KW - leprosy
KW - Male
KW - Molecular Sequence Data
KW - Molecular Weight
KW - Mycobacterium leprae
KW - Protein Sorting Signals
KW - Recombinant Fusion Proteins
KW - Sonication
KW - T-Lymphocytes
AU - Dockrell H M
AU - Stoker N G
AU - Lee S P
AU - Jackson M
AU - Grant K A
AU - Jouy N F
AU - Lucas S B
AU - Hasan R
AU - Hussain R
AU - McAdam K P
AB - <p>The 18-kilodalton (kDa) antigen of Mycobacterium leprae was expressed as a fusion protein with a 2-kDa leader peptide and used in proliferation assays with peripheral blood cells. Fifty percent of untreated tuberculoid leprosy patients and 93% of long-term leprosy contacts responded to the recombinant protein in lymphocyte transformation tests. Comparison of the stimulation indices in the two groups showed that the contacts responded more strongly than the tuberculoid leprosy patients. Seventy percent of Mycobacterium bovis BCG-vaccinated European donors responded, although with low stimulation indices. The isolation of 18-kDa antigen-responsive T-cell lines from a BCG-vaccinated British donor confirmed that the 18-kDa antigen contains at least one cross-reactive epitope. These results indicate that the 18-kDa protein is an important antigen in the immune response to leprosy.</p>
BT - Infection and immunity
C1 - http://www.ncbi.nlm.nih.gov/pubmed/2659530?dopt=Abstract
DA - 1989 Jul
IS - 7
J2 - Infect. Immun.
LA - eng
N2 - <p>The 18-kilodalton (kDa) antigen of Mycobacterium leprae was expressed as a fusion protein with a 2-kDa leader peptide and used in proliferation assays with peripheral blood cells. Fifty percent of untreated tuberculoid leprosy patients and 93% of long-term leprosy contacts responded to the recombinant protein in lymphocyte transformation tests. Comparison of the stimulation indices in the two groups showed that the contacts responded more strongly than the tuberculoid leprosy patients. Seventy percent of Mycobacterium bovis BCG-vaccinated European donors responded, although with low stimulation indices. The isolation of 18-kDa antigen-responsive T-cell lines from a BCG-vaccinated British donor confirmed that the 18-kDa antigen contains at least one cross-reactive epitope. These results indicate that the 18-kDa protein is an important antigen in the immune response to leprosy.</p>
PY - 1989
SP - 1979
EP - 83
T2 - Infection and immunity
TI - T-cell recognition of the 18-kilodalton antigen of Mycobacterium leprae.
VL - 57
SN - 0019-9567
ER -