CD11bGr-1 cells that are increased in the lungs of a Mycobacterium (M) tuberculosis-infection mouse model have the characteristics of monocytic (M)-myeloid-derived suppressor cells (MDSCs) and harbor M.tuberculosis. Interestingly, a high number of M-MDSCs have also been observed in skin lesions of patients with lepromatous leprosy. We hypothesized that CD11bGr-1 cells might be involved in the pathogenesis of leprosy, as they are in tuberculosis. In the current study, we investigated the issue of whether CD11bGr-1 cells accumulate in Mycobacterium (M) leprae-induced granulomas of the footpad skin of nude mice. Our results show that CD11bGr-1 cells began to accumulate in the 7-month-old M.leprae-induced granulomas and were replaced by other leukocytes, including CD11bGr-1 over time during M.leprae infections. CD11b Gr-1 cells expressed the surface markers of M-MDSC, Ly6C and Ly6G. In addition, CD11bGr-1 cells have the nuclei of a mononuclear cell type and expressed higher levels of arginase 1 (Arg1) and inducible NO synthetase (iNOS). Furthermore, they showed a higher infection rate by M.leprae. Taken together, our results indicate that the inoculation with M.leprae induced an accumulation of CD11b Gr-1 at a relatively early stage, 7-month-old M.leprae-induced granulomas, and that CD11bGr-1 have the characteristics of M-MDSC and may act as a reservoir for M.leprae.
BT - Tuberculosis (Edinburgh, Scotland) C1 - https://www.ncbi.nlm.nih.gov/pubmed/37116235 DA - 04/2023 DO - 10.1016/j.tube.2023.102345 J2 - Tuberculosis (Edinb) LA - eng N2 -CD11bGr-1 cells that are increased in the lungs of a Mycobacterium (M) tuberculosis-infection mouse model have the characteristics of monocytic (M)-myeloid-derived suppressor cells (MDSCs) and harbor M.tuberculosis. Interestingly, a high number of M-MDSCs have also been observed in skin lesions of patients with lepromatous leprosy. We hypothesized that CD11bGr-1 cells might be involved in the pathogenesis of leprosy, as they are in tuberculosis. In the current study, we investigated the issue of whether CD11bGr-1 cells accumulate in Mycobacterium (M) leprae-induced granulomas of the footpad skin of nude mice. Our results show that CD11bGr-1 cells began to accumulate in the 7-month-old M.leprae-induced granulomas and were replaced by other leukocytes, including CD11bGr-1 over time during M.leprae infections. CD11b Gr-1 cells expressed the surface markers of M-MDSC, Ly6C and Ly6G. In addition, CD11bGr-1 cells have the nuclei of a mononuclear cell type and expressed higher levels of arginase 1 (Arg1) and inducible NO synthetase (iNOS). Furthermore, they showed a higher infection rate by M.leprae. Taken together, our results indicate that the inoculation with M.leprae induced an accumulation of CD11b Gr-1 at a relatively early stage, 7-month-old M.leprae-induced granulomas, and that CD11bGr-1 have the characteristics of M-MDSC and may act as a reservoir for M.leprae.
PY - 2023 EP - 102345 T2 - Tuberculosis (Edinburgh, Scotland) TI - CD11bGr-1 cells that accumulate in M.leprae-induced granulomas of the footpad skin of nude mice have the characteristics of monocytic-myeloid-derived suppressor cells. UR - https://www.sciencedirect.com/science/article/pii/S1472979223000434/pdfft?md5=ce02808c14239899aafda1ea76893676&pid=1-s2.0-S1472979223000434-main.pdf VL - 140 SN - 1873-281X ER -