TY - JOUR
KW - Antibodies, Monoclonal
KW - Antigens, Differentiation, Myelomonocytic
KW - Blood Platelets
KW - Cell Differentiation
KW - Endothelium
KW - Flow Cytometry
KW - Humans
KW - Immunoenzyme Techniques
KW - Interferon Type I
KW - Interferon-gamma
KW - Macrophages
KW - Molecular Weight
KW - Monocytes
AU - Cabañas C
AU - Sanchez-Madrid F
AU - Bellon T
AU - Figdor C G
AU - Te Velde A A
AU - FERNANDEZ J M
AU - Acevedo A
AU - Bernabeu C
AB - <p>The monoclonal antibody HC1/6 generated against phorbol 12-myristate 13-acetate-treated U-937 cells recognizes a new cell surface antigen with a broad relative molecular mass ranging from 100 to 150 kDa. This antigen is also present on monocytes, platelets and endothelial cells and is weakly expressed by granulocytes. In contrast, it is absent from T, B and erythroblastoid cells. The antigen HC1/6 is also expressed by normal tissue macrophages in tonsil, lung and kidney, as well as in skin biopsies from pathologies such as sarcoidosis and lepromatous leprosy. The expression of the HC1/6 antigen is increased up to 5-fold when U-937 (promonocytic) and HL-60 (myelomonocytic) cell lines are stimulated with phorbol 12-myristate 13-acetate. Conversely, the expression of the HC1/6 antigen is down-regulated in monocytes upon treatment with interferon-gamma. These findings are discussed in relation with other myeloid cell surface markers.</p>
BT - European journal of immunology
C1 - http://www.ncbi.nlm.nih.gov/pubmed/2506060?dopt=Abstract
DA - 1989 Aug
DO - 10.1002/eji.1830190804
IS - 8
J2 - Eur. J. Immunol.
LA - eng
N2 - <p>The monoclonal antibody HC1/6 generated against phorbol 12-myristate 13-acetate-treated U-937 cells recognizes a new cell surface antigen with a broad relative molecular mass ranging from 100 to 150 kDa. This antigen is also present on monocytes, platelets and endothelial cells and is weakly expressed by granulocytes. In contrast, it is absent from T, B and erythroblastoid cells. The antigen HC1/6 is also expressed by normal tissue macrophages in tonsil, lung and kidney, as well as in skin biopsies from pathologies such as sarcoidosis and lepromatous leprosy. The expression of the HC1/6 antigen is increased up to 5-fold when U-937 (promonocytic) and HL-60 (myelomonocytic) cell lines are stimulated with phorbol 12-myristate 13-acetate. Conversely, the expression of the HC1/6 antigen is down-regulated in monocytes upon treatment with interferon-gamma. These findings are discussed in relation with other myeloid cell surface markers.</p>
PY - 1989
SP - 1373
EP - 8
T2 - European journal of immunology
TI - Characterization of a novel myeloid antigen regulated during differentiation of monocytic cells.
VL - 19
SN - 0014-2980
ER -