In this article, we have developed two latex agglutination reaction tests (LAR) with PGL-1 and ND-O-BSA as antigens in 80 cases of leprosy patients (LL = 20, BL = 20, BT = 20, TT = 20), 20 cases of tuberculosis and 25 cases of normal control. These two latex agglutination tests were compared with relevant ELISAs (ND-O-BSA- and PGL-1-ELISA), and analysed by Chi-square (chi 2) test. The results indicated that the sensitivity and specificity of the two LARs (PGL-1- and ND-O-BSA-LAR) are similar to their relevant ELISAs respectively (PGL-1-LAR Vs ND-O-BSA-LAR, chi 2 = 1.785, P greater than 0.10; PGL-1-LAR Vs PGL-1-ELISA, chi 2 = 2.0, P greater than 0.10; ND-O-BSA-LAR Vs ND-O-BSA-ELISA, chi 2 = 0.972, P greater than 0.25), and the increase of positive rate is coincident with clinical classification of leprosy. Since LARs are more simple and rapid than ELISAs, LARs may become more useful tools for immunodiagnosis of leprosy in the field. Also conditions to ensure the stability and reproducibility of LARs are discussed in detail.
BT - Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae C1 - http://www.ncbi.nlm.nih.gov/pubmed/2534621?dopt=Abstract DA - 1989 Oct IS - 5 J2 - Zhongguo Yi Xue Ke Xue Yuan Xue Bao LA - chi N2 -In this article, we have developed two latex agglutination reaction tests (LAR) with PGL-1 and ND-O-BSA as antigens in 80 cases of leprosy patients (LL = 20, BL = 20, BT = 20, TT = 20), 20 cases of tuberculosis and 25 cases of normal control. These two latex agglutination tests were compared with relevant ELISAs (ND-O-BSA- and PGL-1-ELISA), and analysed by Chi-square (chi 2) test. The results indicated that the sensitivity and specificity of the two LARs (PGL-1- and ND-O-BSA-LAR) are similar to their relevant ELISAs respectively (PGL-1-LAR Vs ND-O-BSA-LAR, chi 2 = 1.785, P greater than 0.10; PGL-1-LAR Vs PGL-1-ELISA, chi 2 = 2.0, P greater than 0.10; ND-O-BSA-LAR Vs ND-O-BSA-ELISA, chi 2 = 0.972, P greater than 0.25), and the increase of positive rate is coincident with clinical classification of leprosy. Since LARs are more simple and rapid than ELISAs, LARs may become more useful tools for immunodiagnosis of leprosy in the field. Also conditions to ensure the stability and reproducibility of LARs are discussed in detail.
PY - 1989 SP - 391 EP - 4 T2 - Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae TI - [Study on rapid serodiagnosis for leprosy]. VL - 11 SN - 1000-503X ER -