TY - JOUR
KW - Hypoxic culture
KW - Large-scale production of mycobacteria
KW - Mycobacterium leprae
KW - Mycobacterium tuberculosis
KW - Nontuberculous mycobacteria
KW - Normoxic culture
AU - Wallace E
AU - Hendrickson D
AU - Tolli N
AU - Mehaffy C
AU - Pena M
AU - Nick J
AU - Knabenbaur P
AU - Watkins J
AU - Simpson A
AU - Amin A
AU - Chatterjee D
AU - Dobos K
AU - Lahiri R
AU - Adams L
AU - Strong M
AU - Salfinger M
AU - Bradford R
AU - Stedman T
AU - Riojas M
AU - Hazbón M
AB - <p>Building upon the foundational research of Robert Koch, who demonstrated the ability to grow Mycobacterium tuberculosis for the first time in 1882 using media made of coagulated bovine serum, microbiologists have continued to develop new and more efficient ways to grow mycobacteria. Presently, all known mycobacterial species can be grown in the laboratory using either axenic culture techniques or in vivo passage in laboratory animals. This chapter provides conventional protocols to grow mycobacteria for diagnostic purposes directly from clinical specimens, as well as in research laboratories for scientific purposes. Detailed protocols used for production of M. tuberculosis in large scale (under normoxic and hypoxic conditions) in bioreactors and for production of obligate intracellular pathogens such as Mycobacterium leprae and "Mycobacterium lepromatosis" using athymic nude mice and armadillos are provided.</p>
BT - Methods in molecular biology (Clifton, N.J.)
C1 - https://www.ncbi.nlm.nih.gov/pubmed/34235647
DA - 01/2021
DO - 10.1007/978-1-0716-1460-0_1
J2 - Methods Mol Biol
LA - eng
N2 - <p>Building upon the foundational research of Robert Koch, who demonstrated the ability to grow Mycobacterium tuberculosis for the first time in 1882 using media made of coagulated bovine serum, microbiologists have continued to develop new and more efficient ways to grow mycobacteria. Presently, all known mycobacterial species can be grown in the laboratory using either axenic culture techniques or in vivo passage in laboratory animals. This chapter provides conventional protocols to grow mycobacteria for diagnostic purposes directly from clinical specimens, as well as in research laboratories for scientific purposes. Detailed protocols used for production of M. tuberculosis in large scale (under normoxic and hypoxic conditions) in bioreactors and for production of obligate intracellular pathogens such as Mycobacterium leprae and "Mycobacterium lepromatosis" using athymic nude mice and armadillos are provided.</p>
PY - 2021
SP - 1
EP - 58
T2 - Methods in molecular biology (Clifton, N.J.)
TI - Culturing Mycobacteria.
VL - 2314
SN - 1940-6029
ER -