TY - JOUR
KW - Dermatology
KW - Flow Cytometry
AU - Farag AGA
AU - El Askary SA
AU - Fathy WM
AU - Elbassal F
AU - Azzam AA
AU - Tayel NR
AU - Abdul Karim SS
AU - Shehata WA
AB - Background: Leprosy is a chronic contagious disease caused by Mycobacterium lepraea. CD163 is a monocyte trans-membrane glycoprotein receptor (mCD163) that sheds from the cell surface and circulates as a soluble (serum) form (sCD163). Changes in the mCD163 and sCD163 levels could mirror the categorization of inflammatory procedure, demonstrating a possible use of CD163 as a diagnostic indicator of inflammation.
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Objective: To investigate the possible role of CD163 (sCD163 and mCD163) in leprosy pathogenesis and to assess whether CD163 is a helpful inflammatory marker of leprosy development and typing.
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Patients and Methods: This case control study included 70 leprosy patients and 30 healthy controls. Leprosy patients were classified according to the Madrid criteria (1953) into: tuberculoid leprosy (TT), border-line leprosy (BL), and lepromatous leprosy (LL). For all participants, complete blood count (CBC), serum CD163 using ELISA and monocytes positive for CD163 using flow cytometry were done.
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Results: Leprosy patients had significantly low WBCs and platelet counts (p< 0.001) and had significantly higher sCD163 (p=0.025) and mCD163 (p=0.042) that were highest in LL followed by BL, then TT patients (p< 0.001). There was a significant positive correlation between mCD163 and sCD163 levels in leprosy patients (r=0.896, p< 0.001). ROC analysis revealed a significant role of serum sCD163 and of mCD163 positive monocytes in the detection (p< 0.001) and typing of leprosy (p=0.002 and p< 0.001, respectively).
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Conclusion: Both sCD163 and mCD163 positive monocytes may have an active role in leprosy pathogenesis. They could be potential biomarkers for leprosy detection and typing.
BT - Clinical, Cosmetic and Investigational Dermatology
DO - 10.2147/ccid.s240420
IS - 13
LA - eng
N2 - Background: Leprosy is a chronic contagious disease caused by Mycobacterium lepraea. CD163 is a monocyte trans-membrane glycoprotein receptor (mCD163) that sheds from the cell surface and circulates as a soluble (serum) form (sCD163). Changes in the mCD163 and sCD163 levels could mirror the categorization of inflammatory procedure, demonstrating a possible use of CD163 as a diagnostic indicator of inflammation.
<br>
<br>
Objective: To investigate the possible role of CD163 (sCD163 and mCD163) in leprosy pathogenesis and to assess whether CD163 is a helpful inflammatory marker of leprosy development and typing.
<br>
<br>
Patients and Methods: This case control study included 70 leprosy patients and 30 healthy controls. Leprosy patients were classified according to the Madrid criteria (1953) into: tuberculoid leprosy (TT), border-line leprosy (BL), and lepromatous leprosy (LL). For all participants, complete blood count (CBC), serum CD163 using ELISA and monocytes positive for CD163 using flow cytometry were done.
<br>
<br>
Results: Leprosy patients had significantly low WBCs and platelet counts (p< 0.001) and had significantly higher sCD163 (p=0.025) and mCD163 (p=0.042) that were highest in LL followed by BL, then TT patients (p< 0.001). There was a significant positive correlation between mCD163 and sCD163 levels in leprosy patients (r=0.896, p< 0.001). ROC analysis revealed a significant role of serum sCD163 and of mCD163 positive monocytes in the detection (p< 0.001) and typing of leprosy (p=0.002 and p< 0.001, respectively).
<br>
<br>
Conclusion: Both sCD163 and mCD163 positive monocytes may have an active role in leprosy pathogenesis. They could be potential biomarkers for leprosy detection and typing.
PB - Informa UK Limited
PY - 2020
SP - 379
EP - 389
T2 - Clinical, Cosmetic and Investigational Dermatology
TI - Relationship Between sCD163 and mCD163 and Their Implication in the Detection and Typing of Leprosy
UR - https://www.dovepress.com/front_end/cr_data/cache/pdf/download_1592480001_5eeb510139d78/ccid-240420-relationship-between-scd163-and-mcd163-and-their-implication.pdf
VL - 2020
SN - 1178-7015
ER -